LOCUS       sequence-20735-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(26..150)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(158..174)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(158..174)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(189..630)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(195..218)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    513..630
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(649..668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(650..668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcctgggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
       61 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      121 ttctcgttca gcttttttgt acaaacttgt gatgggggat ccactagttc tagaatccgg
      181 ggttttttct ccttgacgtt aaagtataga ggtatattaa caattttttg ttgatacttt
      241 tattacattt gaataagaag taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg
      301 ttatgcagtt tttgcattta tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt
      361 aattacccca gaaataaggc taaaaaacta atcgcattat catcctatgg ttgttaattt
      421 gattcgttca tttgaaggtt tgtggggcca ggttactgcc aatttttcct cttcataacc
      481 ataaaagcta gtattgtaga atctttattg ttcggagcag tgcggcgcga ggcacatctg
      541 cgtttcagga acgcgaccgg tgaagacgag gacgcacgga ggagagtctt ccttcggagg
      601 gctgtcaccc gctcggcggc ttctaatccg tactagagct ccaattcgcc ctatagtgag
      661 tcgtattaca attcactggc cgtcctttta cacgtcgtga ctg
//
