LOCUS       sequence-207357-        2092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse MeCP2 (deltaN) in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J, De Sousa 
            D, Hector RD, Cobb SR, Bird A
  TITLE     Radically truncated MeCP2 rescues Rett syndrome-like neurological 
            defects.
  JOURNAL   Nature. 2017 Oct 19;550(7676):398-401. doi: 10.1038/nature24058. 
            Epub 2017 Oct 11.
  PUBMED    29019980
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature24058"; journalName: "Nature"; date: "2017-10-19- 
            19"; volume: "550"; issue: "7676"; pages: "398-401"
COMMENT     Sequence Label: pEGFP-N1_MeCP2(deltaN)
FEATURES             Location/Qualifiers
     source          1..2092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             983..1003
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     regulatory      1358..1367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1364..1366,1367..1369,1370..2083)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1364..1366,1367..1369,1370..2080)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1409..1430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1670..1689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2017..2038
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgagcttc gaattctgca attggtcgac ggtaccgcgg gcccgggatc caccggtcgc
       61 caccatggta gctgggatgt tagggctcag ggaggaaaag ccagcagtgc cagaagcctc
      121 ggcttccccc aaacagcggc gctccattat ccgtgaccgg ggacctatgt atgatgaccc
      181 caccttgcct gaaggttgga cacgaaagct caaacaaagg aagtctggcc gatctgctgg
      241 aaagtatgat gtatatttga tcaatcccca gggaaaagct tttcgctcta aagtagaatt
      301 gattgcatac tttgaaaagg tgggagacac ctccttggac cctaatgatt ttgacttcac
      361 ggtaactggg agagggagcc cctccaggag agagcagaaa ccacctaaga agcccaaatc
      421 tcccaaagct ccaggaactg gcaggggtcg gggacgcccc aaagggagcg gcactgggag
      481 accaaaggca gcagcatcag aaggtgttca ggtgaaaagg gtcctggaga agagccctgg
      541 gaaacttgtt gtcaagatgc ctttccaagc atcgcctggg ggtaagggtg agggaggtgg
      601 ggctaccaca tctgcccagg tcatggtgat caaacgccct ggcagaaagc gaaaagctga
      661 agctgacccc caggccattc ctaagaaacg gggtagaaag cctgggagtg tggtggcagc
      721 tgctgcagct gaggccaaaa agaaagccgt gaaggagtct tccatacggt ctgtgcatga
      781 gactgtgctc cccatcaaga agcgcaagac ccgggagacg gtcagcatcg aggtcaagga
      841 agtggtgaag cccctgctgg tgtccaccct tggtgagaaa agcgggaagg gactgaagac
      901 ctgcaagagc cctgggcgta aaagcaagga gagcagcccc aaggggcgca gcagcagtgc
      961 ctcctcccca cctaagaagg agcaccatca tcaccaccat cactcagagt ccacaaaggc
     1021 ccccatgcca ctgctcccat ccccaccccc acctgagcct gagagctctg aggaccccat
     1081 cagcccccct gagcctcagg acttgagcag cagcatctgc aaagaagaga agatgccccg
     1141 aggaggctca ctggaaagcg atggctgccc caaggagcca gctaagactc agcctatggt
     1201 cgccaccact accacagttg cagaaaagta caaacaccga ggggagggag agcgcaaaga
     1261 cattgtttca tcttccatgc caaggccaaa cagagaggag cctgtggaca gccggacgcc
     1321 cgtgaccgag agagttagct gtaaggatcc accggtcgcc accatggtga gcaagggcga
     1381 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1441 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1501 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1561 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1621 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1681 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1741 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1801 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1861 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1921 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1981 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2041 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gc
//
