LOCUS       sequence-207359-        1573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse MeCP2 (deltaNC) in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J, De Sousa 
            D, Hector RD, Cobb SR, Bird A
  TITLE     Radically truncated MeCP2 rescues Rett syndrome-like neurological 
            defects.
  JOURNAL   Nature. 2017 Oct 19;550(7676):398-401. doi: 10.1038/nature24058. 
            Epub 2017 Oct 11.
  PUBMED    29019980
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature24058"; journalName: "Nature"; date: "2017-10-19- 
            19"; volume: "550"; issue: "7676"; pages: "398-401"
COMMENT     Sequence Label: pEGFP-N1_MeCP2(deltaNC)
FEATURES             Location/Qualifiers
     source          1..1573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(845..847,848..850,851..1564)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(845..847,848..850,851..1561)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(890..911)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1151..1170)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1498..1519
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgagcttc gaattctgca attggtcgac ggtaccgcgg gcccgggatc caccggtcgc
       61 caccatggta gctgggatgt tagggctcag ggaggaaaag ccagcagtgc cagaagcctc
      121 ggcttccccc aaacagcggc gctccattat ccgtgaccgg ggacctatgt atgatgaccc
      181 caccttgcct gaaggttgga cacgaaagct caaacaaagg aagtctggcc gatctgctgg
      241 aaagtatgat gtatatttga tcaatcccca gggaaaagct tttcgctcta aagtagaatt
      301 gattgcatac tttgaaaagg tgggagacac ctccttggac cctaatgatt ttgacttcac
      361 ggtaactggg agagggagcc cctccaggag agagcagaaa ccacctaaga agcccaaatc
      421 tcccaaagct ccaggaactg gcaggggtcg gggacgcccc aaagggagcg gcactgggag
      481 accaaaggca gcagcatcag aaggtgttca ggtgaaaagg gtcctggaga agagccctgg
      541 gaaacttgtt gtcaagatgc ctttccaagc atcgcctggg ggtaagggtg agggaggtgg
      601 ggctaccaca tctgcccagg tcatggtgat caaacgccct ggcagaaagc gaaaagctga
      661 agctgacccc caggccattc ctaagaaacg gggtagaaag cctgggagtg tggtggcagc
      721 tgctgcagct gaggccaaaa agaaagccgt gaaggagtct tccatacggt ctgtgcatga
      781 gactgtgctc cccatcaaga agcgcaagac ccgggagacg gtcgggagct ccggcagttc
      841 tggaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      901 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      961 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1021 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1081 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1141 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1201 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1261 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1321 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1381 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1441 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1501 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1561 gtaaagcggc cgc
//
