LOCUS       sequence-20736-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             291..368
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             396..416
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgggct
      241 gcaggaattc gatatcaagc ttatcgatag gtggaccagg tggtggaatg aagcgacgat
      301 ggaaaaagaa tttcatagcc gtctcagcag ccaaccgctt taagaaaatc tcatcctccg
      361 gggcacttga ttatggtacc ccaactactg cttctgaaaa tctatatttt caaggtgaac
      421 taaaaactgc tgctttggct caacatgcga ttaagggtga agctcaaaaa cttaatgact
      481 ctcaagctcc aaaagctgat gcgcaacaaa ataacttcaa caaagatcaa caaagcgcct
      541 tctatgaaat cttgaacatg cctaacttaa acgaagcgca acgtaacggc ttcattcaaa
      601 gtcttaaaga cgacccaagc caaagcacta acgttttaag tgaagctaaa aaattaaacg
      661 aatctcaagc accgaaagct gataa
//
