LOCUS       sequence-207361-        1321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse MeCP2 (deltaNIC) in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J, De Sousa 
            D, Hector RD, Cobb SR, Bird A
  TITLE     Radically truncated MeCP2 rescues Rett syndrome-like neurological 
            defects.
  JOURNAL   Nature. 2017 Oct 19;550(7676):398-401. doi: 10.1038/nature24058. 
            Epub 2017 Oct 11.
  PUBMED    29019980
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature24058"; journalName: "Nature"; date: "2017-10-19- 
            19"; volume: "550"; issue: "7676"; pages: "398-401"
COMMENT     Sequence Label: pEGFP-N1_MeCP2(deltaNIC)
FEATURES             Location/Qualifiers
     source          1..1321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             428..448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(593..595,596..598,599..1312)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(593..595,596..598,599..1309)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(638..659)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(899..918)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1246..1267
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgagcttc gaattctgca attggtcgac ggtaccgcgg gcccgggatc caccggtcgc
       61 caccatggta gctgggatgt tagggctcag ggaggaaaag ccagcagtgc cagaagcctc
      121 ggcttccccc aaacagcggc gctccattat ccgtgaccgg ggacctatgt atgatgaccc
      181 caccttgcct gaaggttgga cacgaaagct caaacaaagg aagtctggcc gatctgctgg
      241 aaagtatgat gtatatttga tcaatcccca gggaaaagct tttcgctcta aagtagaatt
      301 gattgcatac tttgaaaagg tgggagacac ctccttggac cctaatgatt ttgacttcac
      361 ggtaactggg agagggagcc cctccaggag agagcagaaa ccacctggat ccagtggcag
      421 ctctgggccc aagaaaaagc ggaaggtgcc tgggagtgtg gtggcagctg ctgcagctga
      481 ggccaaaaag aaagccgtga aggagtcttc catacggtct gtgcatgaga ctgtgctccc
      541 catcaagaag cgcaagaccc gggagacggt cgggagctcc ggcagttctg gaatggtgag
      601 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      661 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      721 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      781 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      841 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      901 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      961 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1021 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1081 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1141 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1201 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1261 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     1321 c
//
