LOCUS       sequence-207377-        3204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pCK025
FEATURES             Location/Qualifiers
     source          1..3204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..23
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     257..274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             364..1299
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             412..1305
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     primer_bind     complement(1345..1363)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1431..1535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1757..2548
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2719..3204,1..103)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
       61 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      301 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca ttcggctgca
      361 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
      421 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
      481 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
      541 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
      601 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
      661 gcctggctag agatcctgat gattggcctc gtctggcgct ccatggagca cccagggaag
      721 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
      781 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
      841 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
      901 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
      961 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
     1021 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     1081 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     1141 gagatgctgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     1201 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
     1261 tattacatca cgggggaggc agagggtttc cctgccacag tctaggcttt gagaccacga
     1321 agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1381 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1441 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1501 gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa
     1561 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1621 gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     1681 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     1741 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     1801 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     1861 ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac
     1921 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     1981 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     2041 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     2101 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     2161 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     2221 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     2281 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     2341 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     2401 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     2461 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt acgtaaaagg
     2521 cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat atatacttta
     2581 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2641 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2701 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2761 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2821 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2881 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2941 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3001 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3061 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3121 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3181 aggagagcgc acgagggagc ttcc
//
