LOCUS       sequence-207379-        2338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pAGM16841
FEATURES             Location/Qualifiers
     source          1..2338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      36..288
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             591..1382
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1723..2311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2212..2231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctgcact ctgtggtctc attcgtagtc aagcagatcg ttcaaacatt tggcaataaa
       61 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
      121 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
      181 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
      241 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga cgctcgagac
      301 cacgaagtgc cgaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
      361 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      421 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      481 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      541 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
      601 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      661 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      721 cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      781 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      841 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
      901 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
      961 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     1021 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     1081 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     1141 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1201 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1261 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1321 atcagttgga agaatttgtc cattacgtga aaggcgagat caccaaggta gtcggcaaat
     1381 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     1441 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     1501 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgctgtcaga
     1561 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1621 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1681 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1741 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1801 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1861 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1921 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1981 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2041 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2101 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2161 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2221 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2281 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacgg
//
