LOCUS       sequence-207404-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange 
            FP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Starling T, Carlon-Andres I, Iliopoulou M, Kraemer B, 
            Loidolt-Krueger M, Williamson DJ, Padilla-Parra S
  TITLE     Multicolor lifetime imaging and its application to HIV-1 uptake.
  JOURNAL   Nat Commun. 2023 Aug 17;14(1):4994. doi: 10.1038/s41467-023-40731-x.
  PUBMED    37591879
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-17"; volume: "14(1):4994. doi"; pages: " 
            10.1038/s41467-023-40731-x"
COMMENT     Sequence Label: hCXCR4-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggctttca gaccgttaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcttgtc ccctcagttc acctacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cctcaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgactc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg catggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgaggacaa gctcaggctg aagctgaagg acggcggcca ctacacctcc
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagt tgcccggcgc ctacatcgtc
      601 gacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaag
//
