LOCUS       sequence-207426-        7623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Calcium channel beta2a auxillary subunit in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Wyatt CN, Page KM, Berrow NS, Brice NL, Dolphin AC
  TITLE     The effect of overexpression of auxiliary Ca2+ channel subunits on 
            native Ca2+ channel currents in undifferentiated mammalian NG108-15 
            cells.
  JOURNAL   J Physiol. 1998 Jul 15;510 ( Pt 2):347-60.
  PUBMED    9705988
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Physiol."; date: "1998-07-15"; volume: "510 ( Pt 2)"; pages: 
            "347-60"
COMMENT     Sequence Label: cacnb2a (rat) in pMT2 vector
FEATURES             Location/Qualifiers
     source          1..7623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          417..646
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     629..648
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3290..3853
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    4040..4174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4077..4096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4131..4150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4847..4864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5018..5606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5098..5117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5777..6568,6569..6637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6400..6419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6638..6742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6810..6828
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6866..6888)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6988..7007
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7201..7223
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7215..7232
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7216..7232
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7242..7571)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7256..7391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7556..7576
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cgtatagaaa ctcggaccac tctgagacga aggctcgcgt ccaggccagc acgaaggagg
       61 ctaagtggga ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa
      121 gacacatgtc gccctcttcg gcatcaagga aggtgattgg tttataggtg taggccacgt
      181 gaccgggtgt tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct
      241 cttccgcatc gctgtctgcg agggccagct gttgggctcg cggttgagga caaactcttc
      301 gcggtctttc cagtactctt ggatcggaaa cccgtcggcc tccgaacggt actccgccac
      361 cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa cctctcgact gttggggtga
      421 gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga
      481 ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg cgtccatctg
      541 gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc
      601 atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag
      661 gtccaactgc aggcgagcct gaattccggt ttttgtaact tacttgtttt agtatatact
      721 gatctgaaca ggcaaaaccc catgttagca acagctcggt caggaagaga aatgctacct
      781 tttctagatg tttgccggga ggcagccccc tgaaaggaga tctcaaatta cctgtaagaa
      841 aaaaaatttt tttaaaggca gtcagagaga gcaaagtttt ggcatgcctg caaaaacagc
      901 agtgtctgta aaaactacct agagacagca gctaagtgtt gatttgccca tgactcttac
      961 ctgggagtgg aaggtgggaa gaaatggaca gtggccgtga cgagaagctg aggcacggtg
     1021 cagcttggtg aagccacgct ctgactgcgt cctgccacct cttcatgcag tgctgcgggc
     1081 tggtacatcg ccggcgagta cgggtgtcct atggttcggc agactcctac accagccgcc
     1141 catccgattc agatgtgtct ttggaagagg accgggaggc agtccgtaga gaagctgagc
     1201 ggcaggccca ggcccagttg gaaaaagcaa agacaaaacc tgttgcattt gcagttcgga
     1261 ccaatgtcag gtacagcgca gcccaggagg atgacgtccc ggtgcccggc atggccatct
     1321 cattcgaggc aaaagatttt ctgcatgtta aggaaaaatt taataatgac tggtggatag
     1381 gacggctggt taaagaaggc tgtgaaatcg gatttattcc aagcccggtc aaactagaga
     1441 acatgaggct acagcatgaa cagagagcca agcaagggaa attctactcc agtaaatcag
     1501 gaggaaattc atcatccagt ttgggtgaca tagtacccag ttccagaaaa tcgacacctc
     1561 catcatctgc tatagacata gatgctactg gcttagatgc agaagagaat gatattccag
     1621 caaaccaccg ctcccctaag cccagtgcaa acagtgtaac gtcaccccac tccaaagaga
     1681 aaagaatgcc cttctttaag aagacagagc acactcctcc ttacgatgtg gtaccatcca
     1741 tgagaccagt ggtgttggtg ggcccctccc tgaaggggta tgaggtcaca gatatgatgc
     1801 aaaaagcact gtttgatttt ctaaaacaca gatttgaagg acggatatcc atcacaagag
     1861 tcactgctga catctccctg gccaaacgct cagtattaaa caaccccagt aagcacgcaa
     1921 taatagaaag atccaacaca aggtcgagct tagcggaagt tcagagtgaa attgaaagga
     1981 tttttgaact tgcaagaaca ctgcaattgg tagtccttga cgcggataca attaatcacc
     2041 cagctcaact cagtaagacc tctctggccc ctatcatagt gtatgtaaag atttcttctc
     2101 ctaaggtttt acaaaggtta ataaaatctc gaggaaagtc tcaagcaaag cacctcaatg
     2161 tccagatggt ggcagccgat aaactggccc agtgtccccc gcaggagtca tttgatgtga
     2221 tcttggatga gaaccaactt gaggatgctt gtgagcatct cgccgactat ctggaggcgt
     2281 actggaaggc cacccaccct cccagcagta acctccccaa ccctctcctt agccggactt
     2341 tagccacctc aactttacct ctgagcccca cccttgcctc taattcacag ggttctcaag
     2401 gtgatcagag gactgatcgc tctgctcccc ggtctgcttc ccaagctgaa gaagaacctt
     2461 gtctggaacc agtcaaaaaa tcccaacacc gttcttcctc agccacacac caaaaccacc
     2521 gcagtgggac aggtcgaggc ctctctaggc aagaaacgtt tgactctgaa acccaagaga
     2581 gccgagactc tgcctacgtg gagccaaagg aagattattc acatgaacat gtggaccgct
     2641 atgttccaca ccgtgaacat aaccacagag aggagagcca cagcagcaat ggccacaggc
     2701 acagggagcc tcgccaccgc actagggaca tgggtcgaga ccaagaccac aatgagtgca
     2761 gcaaacaacg aagccggcat aaatctaagg atcgctactg tgacaaggaa ggggaagtaa
     2821 tatccaaaag aaggagtgag gctggcgagt ggaacaggga tgtatacatc cgccaatgac
     2881 cgtgcgtgtc tctgccccca agtcttgtgt aacatcatcc ttaagcaaaa tctttggggc
     2941 tccattgcaa ccatctgtga tctgtcttgt atattttgta ttgctgttgc ttgaatagca
     3001 atagcatgca atcaagtatt actatacatt cttttgtaag taccacagaa attggcctag
     3061 tacaagctgc agtcctcagt ctatatcctg aactcttcaa aagctgtttg gggtagctgc
     3121 cacctgaaca gagtggcttc agaaatatag ttaatgtatt cagtgagcca gatgcagcac
     3181 agataacaag tggaattccg gggggggacr gctcagggct gcgatttcgc gccaaacttg
     3241 acggcaatcc tagcgtgaag gctggtagga ttttatcccc gctgccatca tggttcgacc
     3301 attgaactgc atcgtcgccg tgtcccaaaa tatggggatt ggcaagaacg gagacctacc
     3361 ctggcctccg ctcaggaacg agttcaagta cttccaaaga atgaccacaa cctcttcagt
     3421 ggaaggtaaa cagaatctgg tgattatggg taggaaaacc tggttctcca ttcctgagaa
     3481 gaatcgacct ttaaaggaca gaattaatat agttctcagt agagaactca aagaaccacc
     3541 acgaggagct cattttcttg ccaaaagttt ggatgatgcc ttaagactta ttgaacaacc
     3601 ggaattggca agtaaagtag acatggtttg gatagtcgga ggcagttctg tttaccagga
     3661 agccatgaat caaccaggcc acctcagact ctttgtgaca aggatcatgc aggaatttga
     3721 aagtgacacg tttttcccag aaattgattt ggggaaatat aaacttctcc cagaataccc
     3781 aggcgtcctc tctgaggtcc aggaggaaaa aggcatcaag tataagtttg aagtctacga
     3841 gaagaaagac taacaggaag atgctttcaa gttctctgct cccctcctaa agctatgcat
     3901 ttttataaga ccatgggact tttgctggct ttagatcata atcagccata ccacatttgt
     3961 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     4021 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     4081 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4141 caaactcatc aatgtatctt atcatgtctg gatccccggc caacggtctg gtgacccggc
     4201 tgcgagagct cggtgtacct gagacgcgag taagcccttg agtcaaagac gtagtcgttg
     4261 caagtccgca ccaggtactg atatcccacc aaaaagtgcg gcggcggctg gcggtagagg
     4321 ggccagcgta gggtggccgg ggctccgggg gcgaggtctt ccaacataag gcgatgatat
     4381 ccgtagatgt acctggacat ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag
     4441 tcgcggacgc ggttccagat gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc
     4501 tggccggtga ggcgtgcgca gtcgttgacg ctctagaccg tgcaaaagga gagcctgtaa
     4561 gcgggcactc ttccgtggtc tggtggataa attcgcaagg gtatcatggc ggacgaccgg
     4621 ggttcgaacc ccggatccgg ccgtccgccg tgatccatgc ggttaccgcc cgcgtgtcga
     4681 acccaggtgt gcgacgtcag acaacggggg agcgctcctt ttggcttcct tccaggcgcg
     4741 gcggctgctg cgctagcttt tttggcgagc tcgaattaat tctgcattaa tgaatcggcc
     4801 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6721 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     6781 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     6841 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     6901 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6961 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7021 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     7081 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7141 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7201 cccagtcacg acgttgtaaa acgacggcca gtgccaagct ttttgcaaaa gcctaggcct
     7261 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     7321 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     7381 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     7441 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     7501 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     7561 aactgacaca cattccacag gatccggtcg cgcgaatttc gagcggtgtt ccgcggtcct
     7621 cct
//
