LOCUS       sequence-207435-        5745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pCK039
FEATURES             Location/Qualifiers
     source          1..5745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..58)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             452..1243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1414..2002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1903..1922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2156..2173
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt
       61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac
      241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      301 tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc
      481 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
      541 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt
      601 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
      661 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
      721 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
      781 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
      841 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
      901 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
      961 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1021 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1081 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1141 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1201 ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta
     1261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2101 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2161 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2221 gccgattcat taatcactct gtggtctcaa atggatccga ttcgttctcg gacaccttct
     2281 cccgctcgag agctccttcc tggaccacaa ccagatggag tgcaaccaac agctgatagg
     2341 ggtgtatctc ctccagccgg tggacctctt gacggcttac ctgcaaggcg cacaatgtcc
     2401 cgtaccagat tgcccagtcc accagcacca agtccagcgt tttcagcggg cagcttctct
     2461 gacctcctga gacagtttga tccctctttg ttcaacacct cactgtttga ctcacttccg
     2521 ccttttgggg ctcaccatac tgaagccgct actggtgagt gggatgaggt gcagtcaggc
     2581 ttaagagcag ctgatgcccc accacctacg atgagggtgg cagtaactgc tgctaggcca
     2641 ccgagagcta aaccagctcc aagacgcaga gcagcacaac cttccgatgc atctcctgct
     2701 gctcaagtcg atcttcgcac tttagggtat agccagcaac aacaggagaa gatcaaacct
     2761 aaggtccgaa gtacagttgc gcaacaccat gaagcccttg ttggtcatgg gttcactcat
     2821 gcccacatag ttgcactatc ccaacatcct gctgctcttg gaactgttgc ggtgaagtac
     2881 caggacatga ttgctgcttt acctgaagca acacacgagg caatagtcgg tgttggcaaa
     2941 cagtggtctg gcgctagggc tctagaagcc ctccttacag ttgcaggaga attgcgggga
     3001 cctcccttgc agctcgatac cggacaattg ctgaagattg ccaaacgtgg tggtgttacg
     3061 gcagtagaag cagtgcatgc ttggaggaat gctctaactg gagcaccctt gaatctcacg
     3121 ccagaacaag tggtcgctat cgcctccaat attggtggaa aacaagcact agaaactgtc
     3181 caaagattat tgcctgttct ttgtcaggca cacggactta ccccacaaca agtcgttgct
     3241 atagcctcca atggtggagg gaagcaagcg ttagaaacag tgcagcggct actccctgta
     3301 ttatgccagg ctcatggtct aactccacaa caagtggtgg ctatagcctc aaacataggt
     3361 ggtaaacagg cacttgaaac cgtccaaaga ctcctgccgg tcctctgcca ggcacacggc
     3421 ctcacccccg aacaagtggt ggctattgct tcgaatggtg gaggtaagca ggctttagag
     3481 acagtccaga gactactacc cgttctatgc caggcccatg gtttgacccc ggaacaggtt
     3541 gttgctattg cgtcaaatat tggcggcaag caagcgttgg aaaccgttca agcattactc
     3601 cctgttctct gtcaagcaca tgggctaacg cccgagcagg ttgttgcaat tgcatcaaac
     3661 atcggaggaa agcaggcctt agaaacggta caggcacttt taccagtcct ttgccaagca
     3721 cacgggctta cacccgaaca agtggtcgct attgcaagta atataggtgg aaaacaagca
     3781 ctggaaaccg tgcaggcgct tttgccggta ttatgccaag ctcacggcct aactcctgaa
     3841 caggtggttg cgattgcctc acatgatggg ggtaaacagg cactggagac tgtgcagcgg
     3901 cttttgcctg ttttgtgtca agctcatgga ttgacaccag agcaggtggt cgctatagct
     3961 agtcatgatg gaggtaaaca agcgcttgaa accgtgcaac gtctgctgcc agttctatgt
     4021 caagctcatg ggttgacccc acaacaggtt gtagcgatcg cttccaatgg cggaggaaag
     4081 caagctctag aaacggtgca gaggctcctc ccggttcttt gtcaggcgca tggattgacc
     4141 ccggagcagg tggtcgcaat cgccagtaat ataggaggta agcaggcctt ggaaaccgtt
     4201 caggcgttac tcccggttct atgccaggcg catggcctga cccctgaaca ggttgtggcg
     4261 atagccagta acattggggg aaagcaggca cttgaaaccg tacaacgact cctcccagtc
     4321 ctttgtcaag cccacggatt gactccagaa caagtagttg ctatagcttc gcatgatgga
     4381 ggaaagcagg cccttgaaac agttcagcgt cttttgccag tgttgtgtca agcacacgga
     4441 ttgactcctg aacaggttgt cgccattgca tctcacgatg gtggtaagca agctctcgaa
     4501 accgtacagc gactcttgcc tgttctatgc caagcgcatg gcttgacgcc ggaacaggtg
     4561 gtagccatag caagcaacat aggtggcaaa caagctcttg aaacagttca aaggttgtta
     4621 cctgtgcttt gccaagccca cggtttgacc cctcaacagg tggttgctat agcatcaaat
     4681 ggagggggac ggcctgctct tgagacagtg cagcgcctgt tgcccgtgtt gtgtcaagcg
     4741 catggcttaa caccggaaca ggtcgtggca attgcgtcac acgatggcgg caaacaagcg
     4801 ctggaaaccg ttcagcgact cttgcctgtt ctgtgccaag ctcacggtct gacgccccaa
     4861 caggttgttg ccattgcttc aaatggagga gggaggccag cccttgagtc gattgtcgca
     4921 cagctatctc ggcccgaccc tgctttagcc gctctgacaa atgatcatct tgtggctctc
     4981 gcctgcttag gaggtcgccc agctttagac gcagtaaaaa agggtctacc tcatgctccg
     5041 gccttaatca agaggacgaa tcgtagaatc ccagaacgaa cgagccatcg cgtagccgat
     5101 cacgctcaag ttgttagggt tttaggtttt tttcagtgtc attcacatcc ggcacaagct
     5161 ttcgatgatg ccatgaccca gtttggtatg tcaaggcatg gattactgca acttttcaga
     5221 agagtaggag tgacagagct cgaagccaga agcggaactc tgccacccgc tagccaaaga
     5281 tgggatagga tattgcaggc gagtggaatg aagcgcgcga aaccatctcc aacaagcact
     5341 caaaccccgg atcaagcgag tttgcacgct ttcgcagatt ctctcgaacg agatttggat
     5401 gccccttctc caatgcacga aggtgatcaa actagggcga gtagcaggaa gaggtctagg
     5461 agtgatcgtg cagttacggg cccctcagca caacagtctt ttgaggtcag ggtgccagaa
     5521 caaagggacg ctttacatct cccattgtct tggcgtgtaa aaaggccgcg aactagtatt
     5581 ggagggggat taccggaccc agggaccccc actgctgctg atctagctgc ttctagtacg
     5641 gtaatgcgcg agcaagacga ggatccattt gctggggcag ctgatgactt ccccgcattc
     5701 aacgaagaag aattagcatg gttgatggag ttactgccac agtaa
//
