LOCUS       sequence-207437-        5744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pCK038
FEATURES             Location/Qualifiers
     source          1..5744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     103..122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     356..373
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3984..4002)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4070..4174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4396..5187
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(5358..5744,1..202)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
       61 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      121 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      181 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      241 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      301 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      361 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      421 gccgattcat taatcactct gtggtctcaa atggatccga ttcgttctcg gacaccttct
      481 cccgctcgag agctccttcc tggaccacaa ccagatggag tgcaaccaac agctgatagg
      541 ggtgtatctc ctccagccgg tggacctctt gacggcttac ctgcaaggcg cacaatgtcc
      601 cgtaccagat tgcccagtcc accagcacca agtccagcgt tttcagcggg cagcttctct
      661 gacctcctga gacagtttga tccctctttg ttcaacacct cactgtttga ctcacttccg
      721 ccttttgggg ctcaccatac tgaagccgct actggtgagt gggatgaggt gcagtcaggc
      781 ttaagagcag ctgatgcccc accacctacg atgagggtgg cagtaactgc tgctaggcca
      841 ccgagagcta aaccagctcc aagacgcaga gcagcacaac cttccgatgc atctcctgct
      901 gctcaagtcg atcttcgcac tttagggtat agccagcaac aacaggagaa gatcaaacct
      961 aaggtccgaa gtacagttgc gcaacaccat gaagcccttg ttggtcatgg gttcactcat
     1021 gcccacatag ttgcactatc ccaacatcct gctgctcttg gaactgttgc ggtgaagtac
     1081 caggacatga ttgctgcttt acctgaagca acacacgagg caatagtcgg tgttggcaaa
     1141 cagtggtctg gcgctagggc tctagaagcc ctccttacag ttgcaggaga attgcgggga
     1201 cctcccttgc agctcgatac cggacaattg ctgaagattg ccaaacgtgg tggtgttacg
     1261 gcagtagaag cagtgcatgc ttggaggaat gctctaactg gagcaccctt gaatctcacg
     1321 ccagaacaag tggtcgctat cgcctccaat attggtggaa aacaagcact agaaactgtc
     1381 caaagattat tgcctgttct ttgtcaggca cacggactta ccccacaaca agtcgttgct
     1441 atagcctcca atggtggagg gaagcaagcg ttagaaacag tgcagcggct actccctgta
     1501 ttatgccagg ctcatggtct aactccacaa caagtggtgg ctatagcctc aaacataggt
     1561 ggtaaacagg cacttgaaac cgtccaaaga ctcctgccgg tcctctgcca ggcacacggc
     1621 ctcacccccg aacaagtggt ggctattgct tcgaatggtg gaggtaagca ggctttagag
     1681 acagtccaga gactactacc cgttctatgc caggcccatg gtttgacccc ggaacaggtt
     1741 gttgctattg cgtcaaatat tggcggcaag caagcgttgg aaaccgttca agcattactc
     1801 cctgttctct gtcaagcaca tgggctaacg cccgagcagg ttgttgcaat tgcatcaaac
     1861 atcggaggaa agcaggcctt agaaacggta caggcacttt taccagtcct ttgccaagca
     1921 cacgggctta cacccgaaca agtggtcgct attgcaagta atataggtgg aaaacaagca
     1981 ctggaaaccg tgcaggcgct tttgccggta ttatgccaag ctcacggcct aactcctgaa
     2041 caggtggttg cgattgcctc acatgatggg ggtaaacagg cactggagac tgtgcagcgg
     2101 cttttgcctg ttttgtgtca agctcatgga ttgacaccag agcaggtggt cgctatagct
     2161 agtcatgatg gaggtaaaca agcgcttgaa accgtgcaac gtctgctgcc agttctatgt
     2221 caagctcatg ggttgacccc acaacaggtt gtagcgatcg cttccaatgg cggaggaaag
     2281 caagctctag aaacggtgca gaggctcctc ccggttcttt gtcaggcgca tggattgacc
     2341 ccggagcagg tggtcgcaat cgccagtaat ataggaggta agcaggcctt ggaaaccgtt
     2401 caggcgttac tcccggttct atgccaggcg catggcctga cccctgaaca ggttgtggcg
     2461 atagccagta acattggggg aaagcaggca cttgaaaccg tacaacgact cctcccagtc
     2521 ctttgtcaag cccacggatt gactccagaa caagtagttg ctatagcttc gcatgatgga
     2581 ggaaagcagg cccttgaaac agttcagcgt cttttgccag tgttgtgtca agcacacgga
     2641 ttgactcctg aacaggttgt cgccattgca tctcacgatg gtggtaagca agctctcgaa
     2701 accgtacagc gactcttgcc tgttctatgc caagcgcatg gcttgacgcc ggaacaggtg
     2761 gtagccatag caagcaacat aggtggcaaa caagctcttg aaacagttca aaggttgtta
     2821 cctgtgcttt gccaagccca cggtttgacc cctcaacagg tggttgctat agcatcaaat
     2881 ggagggggac ggcctgctct tgagacagtg cagcgcctgt tgcccgtgtt gtgtcaagcg
     2941 catggcttaa caccggaaca ggtcgtggca attgcgtcac acgatggcgg caaacaagcg
     3001 ctggaaaccg ttcagcgact cttgcctgtt ctgtgccaag ctcacggtct gacgccccaa
     3061 caggttgttg ccattgcttc aaatggagga gggaggccag cccttgagtc gattgtcgca
     3121 cagctatctc ggcccgaccc tgctttagcc gctctgacaa atgatcatct tgtggctctc
     3181 gcctgcttag gaggtcgccc agctttagac gcagtaaaaa agggtctacc tcatgctccg
     3241 gccttaatca agaggacgaa tcgtagaatc ccagaacgaa cgagccatcg cgtagccgat
     3301 cacgctcaag ttgttagggt tttaggtttt tttcagtgtc attcacatcc ggcacaagct
     3361 ttcgatgatg ccatgaccca gtttggtatg tcaaggcatg gattactgca acttttcaga
     3421 agagtaggag tgacagagct cgaagccaga agcggaactc tgccacccgc tagccaaaga
     3481 tgggatagga tattgcaggc gagtggaatg aagcgcgcga aaccatctcc aacaagcact
     3541 caaaccccgg atcaagcgag tttgcacgct ttcgcagatt ctctcgaacg agatttggat
     3601 gccccttctc caatgcacga aggtgatcaa actagggcga gtagcaggaa gaggtctagg
     3661 agtgatcgtg cagttacggg cccctcagca caacagtctt ttgaggtcag ggtgccagaa
     3721 caaagggacg ctttacatct cccattgtct tggcgtgtaa aaaggccgcg aactagtatt
     3781 ggagggggat taccggaccc agggaccccc actgctgctg atctagctgc ttctagtacg
     3841 gtaatgcgcg agcaagacga ggatccattt gctggggcag ctgatgactt ccccgcattc
     3901 aacgaagaag aattagcatg gttgatggag ttactgccac agggttcgtg agaccacgaa
     3961 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4021 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4081 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4141 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
     4201 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     4261 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     4321 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     4381 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     4441 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     4501 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
     4561 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     4621 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     4681 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     4741 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     4801 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     4861 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     4921 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     4981 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     5041 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     5101 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     5161 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     5221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5701 agcttggagc gaacgaccta caccgaactg agatacctac agcg
//
