LOCUS       sequence-207438-        3564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG641
FEATURES             Location/Qualifiers
     source          1..3564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             565..798
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(1678..1696)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1764..1868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2090..2881
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3052..3564,1..76)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3541..3560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatggca gatccaatga agctactatc ttctatcgaa
      361 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      421 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtccccg
      481 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      541 ctactgattt ttcctaggtc gagcgccccc ccgaccgatg tcagcctggg ggacgagctc
      601 cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga tttcgatctg
      661 gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga ctccgccccc
      721 tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga tgcccttgga
      781 attgacgagt acggtgggga tccaattcag caagccactg caggagtctc acaagacact
      841 tcggaaaatc ctaacaaaac aatagttcct gctgcattac cacagctcac ccctaccttg
      901 gtgtcactgc tggaggtgat tgaacccgag gtgttgtatg caggatatga tagctctgtt
      961 ccagattcag catggagaat tatgaccaca ctcaacatgt taggtgggcg tcaagtgatt
     1021 gcagcagtga aatgggcaaa ggcgatacca ggcttcagaa acttacacct ggatgaccaa
     1081 atgaccctgc tacagtactc atggatgttt ctcatggcat ttgccctggg ttggagatca
     1141 tacagacaat caagtggaaa cctgctctgc tttgctcctg atctgattat taatgagcag
     1201 agaatgtctc taccctgcat gtatgaccaa tgtaaacaca tgctgtttgt ctcctctgaa
     1261 ttacaaagat tgcaggtatc ctatgaagag tatctctgta tgaaaacctt actgcttctc
     1321 tcctcagttc ctaaggaagg tctgaagagc caagagttat ttgatgagat tcgaatgact
     1381 tatatcaaag agctaggaaa agccatcgtc aaaagggaag ggaactccag tcagaactgg
     1441 caacggtttt accaactgac aaagcttctg gactccatgc atgaggtggt tgagaatctc
     1501 cttacctact gcttccagac atttttggat aagaccatga gtattgaatt cccagagatg
     1561 ttagctgaaa tcatcactaa tcagatacca aaatattcaa atggaaatat caaaaagctt
     1621 ctgtttcatc aaaaatgagc tttgagacca cgaagtggct cttcagtgga cgaaagggcc
     1681 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1861 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1921 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     1981 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2041 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     2101 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2161 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2221 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2281 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2341 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2401 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2461 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2521 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2581 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2641 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2701 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2761 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2821 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     2881 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2941 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3541 gggaaacgcc tggtatcttt atag
//
