LOCUS       sequence-20744-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(19..143)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(256..279)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
       61 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      121 tcagcttttt tgtacaaact tgtgatgggg gatccactaa ttctagaatc cgtccaaact
      181 aagttctggt gttttaaaac taaaaaaaag aataactaaa aaagtaaaat ttaaaaagtt
      241 taagaaataa atttacagaa ttacaatcaa tacctaccgg ccttatatac ttaataatcc
      301 agtaagggaa taatttcagg gaactggttt ccaccttttt tttccgcttt ttccaaatca
      361 aaaaaaacaa aaaggaataa aaaggggaaa aaaatgaaaa aaaaacctgc ctgggtcaat
      421 tggcctgggg caacctttac tccaggcaag gtgcatcact ccattgaagg tgggcccggt
      481 ttttggccgg ttgggccccc ggtccccgga attgccccaa aaaaaaggaa ccaagaactg
      541 aagggtggtg aaaaaaaaca taatttggtg ccgggaatcc ttttttttcc ggaa
//
