LOCUS       sequence-207442-        8532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Snaar-Jagalska
  TITLE     CMV tdTomato-P2A-T2A, blasticidin selectable
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti- V6.3 Ultra-Chili
FEATURES             Location/Qualifiers
     source          1..8532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    128..149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(164..169,170..187,188..194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     207..229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     226..242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     261..281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        263..281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        309..535
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             536..716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    763..888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1381..1614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1799..1843
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2110..2227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2250..2553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2554..2757
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2708..2728
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2754..2778
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2867..2876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2873..3574
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3006..3024)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3274..3293
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3468..3491
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3584..3640
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3662..3715
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3776..3817
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3841..4429
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3894..3914)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4312..4323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4444..4760
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4611..4746
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4808..4855
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4874..5272
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5359..5592
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5664..5785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5701..5720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5755..5774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5825..5960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5980..5999)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5981..5999)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6009..6026)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6009..6025)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6018..6040)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6167..6622
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6254..6273)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6464..6485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6648..6752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6753..6821,6822..7613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6971..6990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7784..8372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8273..8292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(8526..8532,1..11)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
       61 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      241 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
      301 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
      361 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      421 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      481 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      541 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      601 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      661 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      721 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      781 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      841 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      901 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      961 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1021 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1081 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1141 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1201 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1261 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1321 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1381 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1441 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1501 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1561 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1621 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1681 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1741 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1801 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1861 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1921 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1981 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2041 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2101 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2161 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2221 aaattttatc gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2281 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2341 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2401 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2461 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2521 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2581 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2641 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2701 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     2761 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2821 caccgactct agggctgcag gtccgatcca ccggtggatc gatcccgcca ccatggtgag
     2881 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     2941 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3001 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     3061 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     3121 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3181 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     3241 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     3301 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     3361 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     3421 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     3481 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     3541 cctgttcctg tacggcatgg acgagctgta caagggatct ggagcaacaa acttctcact
     3601 actcaaacaa gcaggtgacg tggaggagaa tcccgggcct tctagaggat ccggctccgg
     3661 cgagggcagg ggaagtctac taacatgcgg ggacgtggag gaaaatcccg gcccagctag
     3721 cgtgtgatca gaattcgttc cggagtcgtc gactctagag ggcccgcggt tcgaaggtaa
     3781 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgatcgac
     3841 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3901 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3961 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4021 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4081 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4141 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4201 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4261 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4321 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4381 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggcgatggt
     4441 accggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4501 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4561 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4681 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4741 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4801 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     4861 gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     4921 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     4981 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     5041 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     5101 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     5161 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     5221 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcacaatt
     5281 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5341 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     5401 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5461 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5521 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5581 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     5641 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     5701 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5761 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     5821 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5881 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5941 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6001 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6061 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6361 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6481 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6601 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6721 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6781 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6841 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6901 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6961 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7021 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7081 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7141 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7201 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7261 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7321 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7381 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7441 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7501 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7561 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7621 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7681 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7921 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8461 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8521 agcgcagcga gt
//
