LOCUS       sequence-207445-        9213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cre mediated red to green shift.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Heitzer E, Groenewoud A, Meditz K, Lohberger B, Liegl-Atzwanger B, 
            Prokesch A, Kashofer K, Behrens D, Haybaeck J, Kolb-Lenz D, Koefeler
            H, Riedl S, Schaider H, Fischer C, Snaar-Jagalska BE, de'Jong D, 
            Szuhai K, Zweytick D, Rinner B
  TITLE     Human melanoma brain metastases cell line MUG-Mel1, isolated clones 
            and their detailed characterization.
  JOURNAL   Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.1038/s41598-019-40570-1.
  PUBMED    30858407
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-03-11"; volume: "9(1):4096. doi"; pages: " 
            10.1038/s41598-019-40570-1"
COMMENT     Sequence Label: pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas
FEATURES             Location/Qualifiers
     source          1..9213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..86
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    203..224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(239..244,245..262,263..269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    277..293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     282..304
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     301..317
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     301..317
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     336..356
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        338..356
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        384..610
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             611..791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    838..963
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1456..1689
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1874..1918
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2185..2302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2325..2628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2629..2832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2783..2803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2829..2853
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(2934..2967)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2996..3005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3002..3679
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(3182..3202)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3400..3419
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3594..3617
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(3704..3737)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3788..3797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3794..3796,3797..3799,3800..4510)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3794..3796,3797..3799,3800..4510)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3839..3860)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4100..4119)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4447..4468
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4532..4573
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4597..5185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4650..4670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5068..5079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5200..5516
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5367..5502
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5564..5611
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5630..6028
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6115..6348
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6420..6541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6457..6476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6511..6530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6581..6716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6736..6755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6737..6755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6765..6782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6765..6781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6774..6796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6923..7378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7010..7029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7220..7241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7404..7508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7509..7577,7578..8369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7727..7746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8540..9128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9029..9048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
       61 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      121 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      181 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      241 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      301 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
      361 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
      421 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
      481 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
      541 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
      601 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      781 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      841 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      901 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      961 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1021 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1081 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1141 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1201 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1261 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1321 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1381 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1441 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1501 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1561 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1621 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1681 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1741 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1801 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1861 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1921 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     1981 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2041 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2101 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2161 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     2221 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2281 aattacaaaa attcaaaatt ttatcgataa gcttgggagt tccgcgttac ataacttacg
     2341 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2401 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2461 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2521 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2581 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2641 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2701 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2761 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2821 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2881 gacctccata gaagacaccg actctaggat cactagtcca gtgtggtgga attataactt
     2941 cgtatagcat acattatacg aagttataat tctgcagata tccagcacag tggcggccac
     3001 catggatagc actgagaacg tcatcaagcc cttcatgcgc ttcaaggtgc acatggaggg
     3061 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggcaagc cctacgaggg
     3121 cacccagacc gccaagctgc aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     3181 cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc
     3241 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3301 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggca ccttcatcta
     3361 ccacgtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac
     3421 tctgggctgg gagccctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     3481 gatccacaag gcgctgaagc tgaagggcgg cggccactac ctggtggagt tcaagtcaat
     3541 ctacatggcc aagaagcccg tgaagctgcc cggctactac tacgtggact ccaagctgga
     3601 catcacctcc cacaacgagg actacaccgt ggtggagcag tacgagcgcg ccgaggcccg
     3661 ccaccacctg ttccagtaga gctagctgaa taaggccgct cgaataactt cgtatagcat
     3721 acattatacg aagttattcg agtctagagg gcccgttaga tctctcgagg ttaacgaatt
     3781 cgactacgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3841 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3901 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3961 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4021 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4081 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4141 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4201 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4261 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4321 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4381 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4441 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4501 gctgtacaag ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     4561 tctcgattct acgcgtaccg gttagtaatg atcgacaatc aacctctgga ttacaaaatt
     4621 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4681 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4741 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4801 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4861 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4921 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4981 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5041 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5101 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5161 atctcccttt gggccgcctc cccgcctggc gatggtaccg gtgtggaaag tccccaggct
     5221 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5281 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5341 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5401 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     5461 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5521 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     5581 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc
     5641 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     5701 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     5761 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     5821 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     5881 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     5941 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     6001 gccctctggt tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa
     6061 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6121 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6361 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     6421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6541 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     6601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6721 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6901 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7261 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7321 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     7381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9181 ctcacatgtt ctttcctgcg ttatcccctg att
//
