LOCUS       sequence-207448-       12785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12785)
  AUTHORS   Alpsoy A, Dykhuizen EC
  TITLE     Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its 
            paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes.
  JOURNAL   J Biol Chem. 2018 Jan 26. pii: RA117.001065. doi: 
            10.1074/jbc.RA117.001065.
  PUBMED    29374058
REFERENCE   2  (bases 1 to 12785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Jan 26. pii: RA117.001065. doi: 
            10.1074/jbc.RA117.001065."
COMMENT     Sequence Label: TetO-FUW-BICRAL-FLAG-pgk-puro
FEATURES             Location/Qualifiers
     source          1..12785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3277..3300
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        3326..3825
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3361..3384)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3758..3777
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3846..4445
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3846..3865)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4342..4362
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4469..5057
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4522..4542)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4940..4951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5060..5076)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5061..5077)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5582..5762
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5788..5805)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5794..6018
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6064..6492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6151..6170)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6361..6382
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6501..6521)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6506..6835
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6686..6821
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6748..6767
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6883..6930
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6949..7323
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7453..7574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7490..7509)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7544..7563
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7623..7639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7623..7639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7636..7658)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7647..7663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7671..7677,7678..7695,7696..7701))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7716..7737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7854..7871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8025..8613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8105..8124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8784..9575,9576..9644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9407..9426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9645..9749)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9824..9843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10015..10394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10396..10594
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             10612..10792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10839..10964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11457..11690
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11875..11919
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12217..12334
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    12391..12409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12433..12451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12475..12493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12518..12536
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12560..12578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12602..12620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        12653..12691
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12688..12712
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattgctagc cgatggatcc gtgaattcgc cccatggatg atgatgatga ctcgtgtctc
       61 cttgatctta ttggagaccc acaagcattg aactattttc tacatggacc tagtaataaa
      121 tctagcaatg atgacttgac taatgcagga tattctgcag ccaattcaaa ttcaattttc
      181 gccaactcta gtaatgctga tcctaagtca tccctcaaag gtgtaagcaa ccagcttgga
      241 gaagggccca gtgatggact gccactttca agtagcctcc agtttcttga agatgaactc
      301 gagtcttctc ctcttcctga tctcactgag gaccaacctt tcgacattct tcagaaatcc
      361 ttgcaagagg ccaatatcac tgaacagaca ttggcagaag aggcatattt ggatgccagt
      421 ataggttcaa gccaacagtt tgcacaagct cagcttcatc cttcttcatc agcatccttt
      481 actcaggctt ctaatgtttc taattactca ggtcagacgc tgcagcctat aggggtgacg
      541 catgtgcctg ttggagcatc gtttgcaagc aatacagtgg gtgtacaaca tggctttatg
      601 caacatgtgg ggatcagtgt tcccagccag catttgtcta atagcagtca gattagtggt
      661 tctggtcaaa tacagttaat tgggtcattt ggtaatcatc cttccatgat gactattaat
      721 aacctagatg gatctcaaat catattaaag ggcagcgggc agcaagcccc atcaaatgtg
      781 agtggagggc tcctggttca tagacagact cctaatggca actccttgtt tgggaactct
      841 agttccagtc cagtagcaca gcctgttacc gttccattta acagcacaaa ttttcaaaca
      901 tctttacctg tgcataacat catcatacaa aggggtcttg caccaaattc aaataaagtc
      961 ccaattaata tacagccaaa gcctatccag atgggtcagc aaaatacata caatgtgaac
     1021 aatttgggaa ttcagcagca ccacgtacaa caagggatct cttttgcttc tgcaagctca
     1081 ccccagggct cagtagttgg tccacacatg tctgtgaaca ttgtaaacca acagaacaca
     1141 agaaagccag tcacctcaca ggcagtgagc agcactgggg gcagtattgt tattcattcc
     1201 cccatgggcc aacctcacgc accccaaagt cagttcctta tacctacaag cctttctgtc
     1261 agttccaact cggtacacca cgtccagact ataaatgggc aacttcttca aactcaaccc
     1321 tctcagctca tttctggcca agtggcctca gagcatgtca tgttgaacag aaactcttcc
     1381 aacatgctca ggaccaacca accatatact ggaccgatgc ttaacaacca gaatactgct
     1441 gtccacttag tgtctgggca gacatttgct gcctctggaa gtccagtgat agccaatcat
     1501 gcctctcctc agcttgtggg tggacagatg cccttgcagc aggcatcccc aactgtatta
     1561 cacctgtcac ctgggcagag cagcgtttcc caaggaagac ctggcttcgc caccatgcca
     1621 tcggtgacaa gcatgtcagg acctagtcgg ttccctgctg tcagctcagc cagcactgcc
     1681 catcctagtc ttgggtctgc agttcagtct ggttcatcag gatcaaactt tacaggagat
     1741 cagctgaccc agccaaacag gactccagta ccagtcagtg tgtctcatcg tcttccagtt
     1801 tcttcttcca agtctaccag caccttcagt aacacacctg gaacaggaac ccagcaacaa
     1861 ttcttctgcc aggctcagaa aaaatgtctg aatcagactt cccccatttc tgctcccaag
     1921 accacagacg gcctgaggca agcacagatc cctgggctct tgagcaccac actgccaggg
     1981 caggattctg gaagcaaagt tatatccgca tccttaggaa ccgcacaacc acagcaggaa
     2041 aaagtagttg gatcatctcc tggccatcca gctgtgcagg tggagagtca ttcgggagga
     2101 caaaaaaggc ctgctgcgaa acagctaacg aaaggagctt tcattctcca gcagttgcag
     2161 agggaccaag cccacactgt gacaccagac aaaagtcact tccgatcact aagtgatgcg
     2221 gtacagagac tgctctccta ccacgtgtgc cagggctcca tgcccactga agaagacttg
     2281 agaaaagtgg acaatgaatt tgagacagtt gccactcagc tcctaaaaag gacccaagct
     2341 atgcttaaca aatacagatg cctgctccta gaagatgcca tgcgaatcaa tccctctgct
     2401 gagatggtga tgatcgatag gatgttcaac caggaggaaa gagcttccct gtcccgagac
     2461 aagcgtttgg cacttgtaga ccctgagggt tttcaggctg atttctgttg ttccttcaaa
     2521 cttgataaag ctgctcatga gacacagttt ggccggagtg accagcatgg cagtaaagca
     2581 agcagctctc tgcaaccgcc agccaaggcc caaggcagag accgagccaa aaccggtgtg
     2641 acggaaccca tgaatcatga ccagtttcat ctagtgccta atcacatcgt ggtctctgca
     2701 gaaggaaaca tttctaaaaa aacagaatgc cttggcagag cactgaaatt tgacaaagtg
     2761 ggcttagtgc agtaccagag cacgtctgaa gagaaggcca gccggagaga gcctctgaag
     2821 gccagtcagt gctctcccgg ccctgagggg caccggaaaa cctcatccag atcggatcat
     2881 ggtactgaga gcaaactgtc aagcatccta gcagattcgc acttggagat gacgtgtaac
     2941 aattccttcc aggacaaaag tctgaggaat tctccaaaga atgaagtttt acacacagac
     3001 atcatgaaag ggtcaggcga accccagcca gatctccagc tgacaaagag cttggaaacc
     3061 acatttaaga acatcttgga actcaaaaag gcgggacggc agccccagag tgaccccacg
     3121 gttagcggct ctgttgagtt agatttcccc aacttttctc ctatggcttc acaggaaaac
     3181 tgcctggaaa agttcatccc ggaccacagt gaaggtgttg tagaaactga ctccatttta
     3241 gaagcagctg taaatagtat cctagagtgt ggcggagact acaaagacga tgacgacaag
     3301 tgatgagaat tccataccgg ttaccgggta ggggaggcgc ttttcccaag gcagtctgga
     3361 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     3421 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     3481 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     3541 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     3601 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     3661 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     3721 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     3781 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     3841 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     3901 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     3961 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4021 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4081 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4141 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4201 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4261 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4321 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4381 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4441 cctgaaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     4501 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4561 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4621 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4681 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4741 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4801 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4861 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4921 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4981 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5041 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     5101 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     5161 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     5221 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5281 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5341 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     5401 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     5461 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     5521 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     5581 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5641 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5701 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5761 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5821 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5881 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5941 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6001 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6061 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6121 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6181 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6241 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6301 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6361 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6421 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6481 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6541 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6601 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6661 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6721 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6781 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6841 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     6901 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     6961 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     7021 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     7081 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     7141 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     7201 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     7261 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     7321 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7381 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7441 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7501 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7561 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     7621 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7681 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8761 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8821 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8881 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8941 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9001 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9061 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9121 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9181 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9241 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9301 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9361 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9421 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9481 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9541 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9601 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9661 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9721 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9781 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     9841 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     9901 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     9961 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
    10021 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    10081 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    10141 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    10201 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    10261 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    10321 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    10381 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
    10441 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
    10501 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
    10561 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
    10621 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10681 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10741 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    10801 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    10861 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    10921 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    10981 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    11041 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    11101 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    11161 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    11221 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    11281 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
    11341 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11401 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11461 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    11521 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    11581 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    11641 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    11701 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    11761 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    11821 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    11881 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    11941 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    12001 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    12061 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    12121 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
    12181 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
    12241 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    12301 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
    12361 tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
    12421 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
    12481 tcagtgatag agaaaagtga aagtcgaggt ttaccactcc ctatcagtga tagagaaaag
    12541 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
    12601 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
    12661 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
    12721 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
    12781 ccccg
//
