LOCUS       sequence-20745-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(119..243)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             302..379
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             407..427
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cggggaagag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
       61 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      121 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      181 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      241 ggtgatgggc tgcaggaatt cgatatcaag cttatcgata ggtggaccag gtggtggaat
      301 gaagcgacga tggaaaaaga atttcatagc cgtctcagca gccaaccgct ttaagaaaat
      361 ctcatcctcc ggggcacttg attatggtac cccaactact gcttctgaaa atctatattt
      421 tcaaggtgaa ctaaaaactg ctgctttggc tcaacatgcg attaagggtg aagctcaaaa
      481 acttaatgac tctcaagctc caaaagctga tgcgcaacaa aataacttca acaaagatca
      541 acaaagcgcc ttctatgaaa tcttgaacat gcctaactta aacgaagcgc aacgtaacgg
      601 cttcattcaa agtcttaaag acgacccaag ccaaagcact aacgttttag gtga
//
