LOCUS       sequence-207450-        4995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transgenesis vector based on the Minos transposon, carrying the 
            3xP3-DsRed marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Pavlopoulos A, Averof M
  TITLE     Establishing genetic transformation for comparative developmental 
            studies in the crustacean Parhyale hawaiensis.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 May 31;102(22):7888-93. Epub 2005 May
            23.
  PUBMED    15911760
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-05-31"; volume: "102(22)"; pages: 
            "7888-93. Epub 2005 May 23"
COMMENT     Sequence Label: pMi(3xP3-DsRed)
FEATURES             Location/Qualifiers
     source          1..4995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     471..487
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     472..488
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      773..782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             779..1456
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(909..927)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(959..979)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1177..1196
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1371..1394
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1577..1698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1614..1633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1668..1687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1735..1751)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1735..1751)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2153..2172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2154..2172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2179..2196)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2179..2195)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2188..2210)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2337..2792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2424..2443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2634..2655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2818..2922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2923..2991,2992..3783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3141..3160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3954..4542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4443..4462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4696..4713
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4830..4851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4866..4871,4872..4889,4890..4896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4904..4920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4909..4931
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4928..4944
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4928..4944
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4965..4983
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gtaccaagtg cttgaaatgc taaatgtttt caatttttcg ccattaagac aagcctacac
       61 aaatgcttct ataaattatg ccaagcacgt tagcagcttc tacgagcccc aaccactatt
      121 aattcgaaca gcatgttttt ttgcagtgcg caatgtttaa cacactatat tatcaatact
      181 actaaagata acacatacca atgcatttcg tctcaaagag aattttattc tcttcacgac
      241 gaaaaaaaaa gttttgctct atttccaaca acaacaaaaa tatgagtaat ttattcaaac
      301 ggtttgctta agagataaga aaaaagtgac cactattaat tcgaacgcgg cgtaagctta
      361 ccttaatctc aagaagagca aaacaaaagc aactaatgta acggaatcat tatctagtta
      421 tgatctgcaa ataatgctgc agagctgggg gatccccggc gggccccccc tcgaggtcga
      481 cggtatcgat aagcttgata tcgaattcga gctcgcccgg ggatctaatt caattagaga
      541 ctaattcaat tagagctaat tcaattagga tccaagctta tcgatttcga accctcgacc
      601 gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa
      661 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
      721 aatcggggta ccgctagagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
      781 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
      841 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      901 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
      961 gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga
     1021 ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     1081 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggctcct tcatctacaa
     1141 ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga agaagactat
     1201 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     1261 ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta
     1321 catggccaag aagcccgtgc agctgcccgg ctactactac gtggactcca agctggacat
     1381 cacctcccac aacgaggact acaccatcgt ggagcagtac gagcgcgccg agggccgcca
     1441 ccacctgttc ctgtagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     1501 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1561 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1621 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1681 actcatcaat gtatcttaaa gcttatcgat acgcgtacgg cgcgcctagg gggggatcca
     1741 ctagttctag agcggccgcc accgcgggac gttacacaat tctaatatta attaaattat
     1801 tgttttaagt atgatagtaa atcacattac gccgcgttcg aattaatagt ggtcactttt
     1861 ttcttatctc ttaagcaaac cgtttgaata aattactcat atttttgttg ttgttggaaa
     1921 tagagcaaaa cttttttttt cgtcgtgaag agaataaaat tctctttgag acgaaatgca
     1981 ttggtatgtg ttatctttag tagtattgat aatatagtgt gttaaacatt gcgcactgca
     2041 aaaaaaacat gctgttcgaa ttaatagtgg ttggggctcg tagaaaacga aaaatatctt
     2101 aagctagcat agagaatgga gcaaaactca atttgatgcg agctccaatt cgccctatag
     2161 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2221 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2281 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     2341 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2401 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2461 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2521 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2581 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2641 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2701 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2761 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     2821 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2881 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2941 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3001 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3061 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3121 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3181 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3241 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3301 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3361 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3421 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3481 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3541 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3601 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3661 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3721 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3781 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3841 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3901 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3961 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4021 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4081 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4141 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4201 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4261 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4321 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4381 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4441 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4501 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4561 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4621 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4681 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4741 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4801 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4861 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4921 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
     4981 agggaacaaa agctg
//
