LOCUS       sequence-207458-       10417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) 
            gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Marshall JM, Buchman A, Sanchez C HM, Akbari OS
  TITLE     Overcoming evolved resistance to population-suppressing homing-based
            gene drives.
  JOURNAL   Sci Rep. 2017 Jun 19;7(1):3776. doi: 10.1038/s41598-017-02744-7.
  PUBMED    28630470
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-06-19"; volume: "7(1):3776. doi"; pages: " 
            10.1038/s41598-017-02744-7"
COMMENT     Sequence Label: OA-16
FEATURES             Location/Qualifiers
     source          1..10417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(19..447)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(129..150)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     341..360
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     574..596
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     588..605
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     589..605
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     612..631
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        612..630
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(664..682)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1134..1205,1607..1880,1955..2609,2671..2986,3190..3321,
                     3392..4006)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4781..4814
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        6801..6876
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           6881..6948
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        7014..7089
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           7094..7161
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     intron          7239..7304
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7434..7454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7726..7847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7763..7782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7817..7836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    7930..7999
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8218..8236)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8257..8273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8257..8273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8270..8292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8281..8297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8305..8311,8312..8329,8330..8335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8350..8371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8488..8505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8659..9247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8739..8758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9418..10209,10210..10278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10041..10060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10279..10383)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
       61 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
      121 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
      181 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
      241 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
      301 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
      361 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
      421 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
      481 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
      541 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
      601 ccagtgaatt gtaatacgac tcactatagg gcgaattggg tacgtaccgg gcccctagta
      661 tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac attttttttt
      721 tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact tcgatccaag
      781 ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat tccactcaaa
      841 gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt tcgccgctgc
      901 gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt
      961 tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc aattaaacac
     1021 aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg agtgattggg
     1081 gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc
     1141 aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc gagcatctga
     1201 acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag gaactgcgat
     1261 tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat
     1321 tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg ctatataaga
     1381 caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc gactatattc
     1441 ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc acaaaaaata
     1501 aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc ggggttagat
     1561 gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg acagcggagc
     1621 ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg gcacgctccg
     1681 gccacccagt ccgccggagg actccggttc agggagcggc caactagccg agaacctcac
     1741 ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct ccggatggcg
     1801 gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac cggcgcccag
     1861 gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg tgtgaaatct
     1921 taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa
     1981 cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc
     2041 tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct caatggccaa
     2101 cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga tgacctcttt
     2161 atcggctccc taacggccag ggaacacctg attttccaag ccatggtgcg gatgccacga
     2221 catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga gctttcgctc
     2281 agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct gtccggcgga
     2341 gaaaggaagc gtctggcatt cgcctccgag gctctaaccg atccgccgct tctgatctgc
     2401 gatgagccca cctccggact ggactccttt accgcccaca gcgtcgtcca ggtgctgaag
     2461 aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc ttccgagctg
     2521 tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt cttgggcact
     2581 cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta agggtatcta
     2641 gtattacata acatctcaac tcctatccag cgtgggtgcc cagtgtccta ccaactacaa
     2701 tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg
     2761 tgatcggatc gccaagatat gcgacaattt tgccattagc aaagtagccc gggatatgga
     2821 gcagttgttg gccaccaaaa atctggagaa gccactggag cagccggaga atgggtacac
     2881 ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt
     2941 gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag
     3001 tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagatag
     3061 aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt
     3121 gcagaagata aataaatttc atttaactcg cgaatattaa tgagatgcga gtaacatttt
     3181 aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa
     3241 gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa
     3301 aacgtctttg ccacgataaa tgtaagtcat gtttagaata catttgcatt tcaataattt
     3361 actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat
     3421 gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc
     3481 cgaattgccg ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat
     3541 cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc
     3601 caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc
     3661 gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa
     3721 ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc
     3781 caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac
     3841 atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc
     3901 cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt
     3961 gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg
     4021 aaataactgc ttgttttttt tttttaccat tattaccatc gtgtttactg tttattgccc
     4081 cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat
     4141 acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa
     4201 agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg
     4261 gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac
     4321 cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca
     4381 ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa
     4441 atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca
     4501 atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta
     4561 atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt
     4621 tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg
     4681 ggcataatag tgttgtttat atatatcaaa aataacaact ataataataa gaatacattt
     4741 aatttagaaa atgcttggat ttcactggaa ctaggctagc ataacttcgt ataatgtatg
     4801 ctatacgaag ttatgctagc ggatccggga attgggaatt gggcaatatt taaatggcgg
     4861 ccgcgcgcag atcgccgatg ggcgtggcgc cggagggcac cttcaccttg ccgtacggct
     4921 tgctgttctt cgcgttcaaa atctccagct ccattttgct ttcggtgcgc ttgcaatcag
     4981 tactgtccaa aatcgaaaat cgccgaaccg tagtgtgacc gtgcggggct ctgcgaaaat
     5041 aaactttttt aggtatatgg ccacacacgg ggaaagcaca gtggattata tgttttaata
     5101 ttataatatg caggttttca ttacttatcc agatgtaagc ccacttaaag cgatttaaca
     5161 attatttgcc gaaagagtat aaacaaattt cacttaaaaa tggattaaga aaagcttgtg
     5221 taagattatg cgcagcgttg ccagatagct ccatttaaaa cacttcaaaa acaataagtt
     5281 ttgaaaatat atacataaat agcagtcgtt gccgcaacgc tcaacacatc acacttttaa
     5341 aacacccttt acctacacag aattactttt taaatttcca gtcaagctgc gagtttcaaa
     5401 attatagccg gtagagaaga cagtgctatt tcaaaagcaa actaacaagg gtcttaaatt
     5461 ccaaaacacc aatcctaaca agccttggac ttttgtaagt ttagatcaaa ggtggcattg
     5521 cattcaatgt catggtaaga agtaggtcgt ctaggtagaa atcctcattc agccggtcaa
     5581 gtcagtacga gaaaggtctc aatttgaaat tgtcttaaaa atattttatt gttttgtact
     5641 gtggtgagtt taaacgaaaa acacaaaaaa aaagtgatac acagaaatca taaaaaattt
     5701 taatacaagg tattcgtacg tatcaaaaac atttcggcac aatttttttt ctctgtacta
     5761 aagtgttacg aacactacgg tattttttag tgattttcaa cggacaccga aggtatataa
     5821 acagcgttcg cgaacggtcg ccttcaaaac caattgacat ttgcagcagc aagtacaagt
     5881 agaaagtaaa gcgcaatcag cgaaaaattt atacttaatt gttggtgatt aaagtacaat
     5941 taaaagaaca ttctcgaaag tcacaagaaa cgtaagtttt taactcgctg ttaccaatta
     6001 gtaataagag caacaagacg ttgagtaatt tcaagaaaaa ctgcatttca aggtctttgt
     6061 tcggccattt tttttttatt caacgctcta cgtaattaca aaataagaaa ttggcagcca
     6121 cgcatcttgt tttcccaatg aattggcatc aaaacgcaaa caaatctata aataaaactt
     6181 gcgtgttgat tttcgccaag atttattggc aaattgtgaa attcgcagtg acgcatttga
     6241 aaattcgaga aatcacgaac gcactcgagc atttgtgtgc atgttattag ttagttgttt
     6301 agttaattga agtattttac caacgaaatc cacttatttt tagctgaaat agagtaggtt
     6361 gcttaaacaa agccacgtct gaaaatttct tattgcttgt agttgtgacg tcaccatata
     6421 cacacaaaat aatgtgtatg catgcatttc agctgtgtat atatacatgc acacactcgc
     6481 aacacgaaaa cgatgacgaa gcaacggaac aaaggtttct caactaccct ttgttccctg
     6541 tttcttcgct ttcctttgtt ccaatattcg tagagggtta ataggggttt ctcaacaaag
     6601 ttggcgtcga taaataagtt tcccattttt attccccagc caggaagtta gtttcaatag
     6661 ttttgtaatt tcaacgaaac tcatttgatt tcgtactaat tttccacatc tctattttga
     6721 cccgcagagg cgcgccatcg ccctgatgag tcccgtgagg acgaaacgag taagctcgtc
     6781 ggcgatactt ggatgccctg gttttagagc tagaaatagc aagttaaaat aaggctagtc
     6841 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ggccggcatg gtcccagcct
     6901 cctcgctggc gccggctggg caacatgctt cggcatggcg aatgggacaa aaaccctgat
     6961 gagtcccgtg aggacgaaac gagtaagctc gtcggttttg gacactggaa ccggttttag
     7021 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     7081 agtcggtgct tttggccggc atggtcccag cctcctcgct ggcgccggct gggcaacatg
     7141 cttcggcatg gcgaatggga cagatctttg tgaaggaacc ttacttctgt ggtgtgacat
     7201 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     7261 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     7321 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     7381 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     7441 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     7501 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     7561 ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc ttgactagag
     7621 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     7681 ctccccctga acctgaaaca taaaatgaat ggaattgttg ttgttaactt gtttattgca
     7741 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7801 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggttc
     7861 cattaattaa gaattcggcc gcaacggtac ctctagagat ccactagtgt cgacgatgta
     7921 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     7981 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
     8041 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
     8101 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
     8161 cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct
     8221 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8281 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8341 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8401 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8461 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8521 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8581 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8641 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8701 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8761 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8821 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8881 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8941 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9001 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9061 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9121 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9181 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9241 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9301 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9361 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9421 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9481 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9541 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9601 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9661 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9721 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9781 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9841 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9901 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9961 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10021 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10081 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10141 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10201 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10261 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10321 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10381 gcgcacattt ccccgaaaag tgccacctaa attgtaa
//
