LOCUS       sequence-207461-        3813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pCK26
FEATURES             Location/Qualifiers
     source          1..3813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1927..1945)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2013..2117
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2339..3130
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3301..3813,1..76)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3790..3809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatgatg aatcagaatt gctttaattc ttgttcacct
      361 ctaactgttg atgcacttga accaaaaaaa tcctcttgtg ctgctaaatg catacaagta
      421 aatggtcctc ttattgttgg agctggccct tcaggcctgg ctactgctgc cgtccttaag
      481 caatacagtg ttccgtatgt aatcattgaa cgcgcggact gcattgcttc tctgtggcaa
      541 cacaagacct acgatcggct taggcttaac gtgccacgac aatactgcga attgcctggc
      601 ttgccatttc caccagactt tccagagtat ccaaccaaaa accaattcat cagctacctc
      661 gtatcttatg caaagcattt cgagatcaaa ccacaactca acgagtcagt aaacttagct
      721 ggatatgatg agacatgtgg tttatggaag gtgaaaacag tttctgaaat caatggttca
      781 acctctgaat acatgtgtaa gtggcttatt gtggccacag gagagaatgc tgagatgata
      841 gtgcccgaat tcgaaggatt gcaagatttt ggtggccagg ttattcatgc ttgtgagtac
      901 aagactgggg aatactatac tggagaaaat gtgctggcgg ttggctgtgg caattccggg
      961 atcgatatct cacttgatct ttcccaacat aatgcaaatc cattcatggt agttcgaagc
     1021 tcggtaagtt ttatattcaa taagtattat ttttcaagta acactagaaa gtgatcttgt
     1081 atctttcatt tgctcgcatg aatatattat attcacacat gaatgatatc atctagtttt
     1141 gttaatcttt caggtacagg gtcgtaattt ccctgaggaa ataaacatag ttccagcaat
     1201 caagaaattt actcaaggaa aagtagaatt tgttaatgga caaattctag agatcgactc
     1261 tgttatcttg gcaactggtt ataccagcaa tgtaacttct tggttaatgg taaggaaata
     1321 cacaagtttt atttctatgc ctaattaaat tggtgtttaa tcataaatta tatatagtac
     1381 taagtatgat aaaagctcct tcaactataa aggatgattt agtcaaatga actcttaatg
     1441 aatgtagtaa ttatttatgg attcttgtta cattcatgta agttggtatc tcattatcct
     1501 gtggattctt tcctttgagt tattaattag ttagaattca ctataaccgt cttttttctt
     1561 ttaccctttc ctcatacctt tttgttcttt tgataactcg aactcacaat cttaagattg
     1621 ggaataaggg gctctttacc atctgagcaa ctttctctcg ttctataata gcccccttcg
     1681 aaatttggtc taatgagaat tttactgata caggagagtg aattgttttc aagggaggga
     1741 tgtccaaaaa gcccattccc aaatggttgg aagggggagg atggtctgta tgcagttgga
     1801 tttacaggaa taggactgtt tggtgcttct atagatgcca ctaatgttgc acaagatatt
     1861 gccaaaattt ggaaagaaca aatgtaggct ttgagaccac gaagtggctc ttcagtggac
     1921 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1981 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2041 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2101 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     2161 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca
     2221 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2281 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     2341 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2401 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2461 cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2521 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2581 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2641 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2701 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2761 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2821 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     2881 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     2941 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3001 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3061 gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt
     3121 cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3241 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3781 gcttccaggg ggaaacgcct ggtatcttta tag
//
