LOCUS       sequence-207462-        4773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for synthesis of Minos transposase mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Pavlopoulos A, Berghammer AJ, Averof M, Klingler M
  TITLE     Efficient transformation of the beetle Tribolium castaneum using the
            Minos transposable element: quantitative and qualitative analysis of
            genomic integration events.
  JOURNAL   Genetics. 2004 Jun;167(2):737-46.
  PUBMED    15238525
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2004-06"; volume: "167(2)"; pages: "737-46"
COMMENT     Sequence Label: pBlueSKMimRNA
FEATURES             Location/Qualifiers
     source          1..4773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     712..733
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(2585..2605)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2585..2603)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2624..2640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2624..2640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2637..2659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2648..2664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2672..2678,2679..2696,2697..2702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2717..2738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2855..2872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3026..3614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3106..3125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3785..4576,4577..4645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4408..4427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4646..4750)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaaagt
      661 gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact
      721 gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctggggga tctgatcgat
      781 aatggttcgt ggtaaaccta tttctaaaga aatcagagta ttgattaggg attattttaa
      841 atctggaaag acacttacgg agataagcaa gcaattaaat ttgcctaagt cgtctgtgca
      901 tggggtgata caaattttca aaaaaaatgg gaatattgaa aataacattg cgaatagagg
      961 ccgaacatca gcaataacac cccgcgacaa aagacaactg gccaaaattg ttaaggctga
     1021 tcgtcgccaa tctttgagaa atttggcttc taagtggtcg cagacaattg gcaaaactgt
     1081 caagcgagag tggacgcgac agcaattaaa aagtattgga tatggttttt ataaagccaa
     1141 ggaaaaaccc ttgcttacgc ttcgtcaaaa aaagaagcgt ttgcaatggg ctcgggaaag
     1201 gatgtcttgg actcaaaggc aatgggatac catcatattc agcgatgaag ctaaatttga
     1261 tgttagtgtc ggcgatacga gaaaacgcgt catccgtaag aggtcagaaa cataccataa
     1321 agactgcctt aaaagaacaa caaagtttcc tgcgagcact atggtatggg gatgtatgtc
     1381 tgccaaagga ttaggaaaac ttcatttcat tgaagggaca gttaatgctg aaaaatatat
     1441 taatatttta caagatagtt tgttgccatc aataccaaaa ctatcagatt gcggtgaatt
     1501 cacttttcag caggacggag catcatcgca cacagccaag cgaaccaaaa attggctgca
     1561 atataatcaa atggaggttt tagattggcc atcaaatagt ccagatctaa gcccaattga
     1621 aaatatttgg tggctaatga aaaaccagct tcgaaatgag ccacaaagga atatttctga
     1681 cttgaaaatc aagttgcaag agatgtggga ctcaatttct caagagcatt gcaaaaattt
     1741 gttaagctca atgccaaaac gagttaaatg cgtaatgcag gccaagggcg acgttacaca
     1801 attctaatct agtcgacggt atcgatgtta taagttctga accgagaaag aggggagaat
     1861 gtagagccga tagctgccca atactgccac gcccccagca gcccaggcat ggaaaataat
     1921 acagagaaaa tccacactta ttaggtgcta ctgtaatttt ttttaaatcg cttaaaatta
     1981 ttcggcgttt gcctagtgca aggcccattt atggacaatt gtttgccaaa ctcaaagttt
     2041 gccggtcgga ggaaaagtta caacgccaaa atgtgttgaa aaatgcaaaa gaaattatta
     2101 aaaacattaa aaaattatta aataatctat gtaattcttg aatgtaaact ctgtgtaaat
     2161 actaaaaatg aaaaacaaaa tggaaagcgt agatggagaa cagttgaatc gttatatatt
     2221 taaattaata ttgcatttat ttccaaatta cagtgggaga agcgctgacc gacttcttgc
     2281 agtgtactct cgaaataaaa cccctgtctg taccacgtaa aacccctact aaaaattagt
     2341 ctcgcccgct gctcacagtt agtttagttc aattatacgc tactgtttaa tttattaact
     2401 gtacttatta atgcgcgtca tgaataatga ttgactgtcc cttatttttt ttttttgcta
     2461 atttctgtat taaatttaat gatttaccat aaaaatataa ataaaaatga cggtcgagtt
     2521 gtaaaattaa aaaaaaaaaa cgaatgctgc ggccgccacc gcggtggagc tccagctttt
     2581 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     2641 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2701 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2761 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2821 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2881 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2941 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3001 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3061 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4741 gggttccgcg cacatttccc cgaaaagtgc cac
//
