LOCUS       sequence-207463-        3467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for easy cloning into Minos/3xP3 transgenesis 
            vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Horn C, Wimmer EA
  TITLE     A versatile vector set for animal transgenesis.
  JOURNAL   Dev Genes Evol. 2000 Dec;210(12):630-7.
  PUBMED    11151300
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Genes 
            Evol."; date: "2000-12"; volume: "210(12)"; pages: "630-7"
COMMENT     Sequence Label: pSLfa1180fa
FEATURES             Location/Qualifiers
     source          1..3467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(344..361)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(344..360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(353..375)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      573..1028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(660..679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     870..891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1045..1064)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1164..1186
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1224..1242)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1310..1414
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1415..1483,1484..2275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1633..1652)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2446..3034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2935..2954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3188..3205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3322..3343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3358..3363,3364..3381,3382..3388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3396..3412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3401..3423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3420..3436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3420..3436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcgaat ggccatggga cgtcgacctg aggtaattat aacccgggcc ctatatatgg
       61 atccaattgc aatgatcatc atgacagatc tgcgcgcgat cgatatcagc gctttaaatt
      121 tgcgcatgct agctatagtt ctagaggtac cggttgttaa cgttagccgg ctacgtatac
      181 tccggaatat taataggcct aggatgcata tggcggccgc ctgcagctgg cgccatcgat
      241 acgcgtacgt cgcgaccgcg gacatgtaca gagctcgaga agtactagtg gccacgtggg
      301 ccgtgcacct taagcttggc gcgcctaggc cggccagctt ggcactggcc gtcgttttac
      361 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      421 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      481 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      541 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
      601 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      661 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      721 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      781 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      841 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      901 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
      961 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     1021 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     1081 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1141 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1201 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1261 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1321 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1381 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1441 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1501 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1561 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1621 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1681 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1741 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1801 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1861 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1921 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1981 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2041 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2101 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2161 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2221 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2281 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2341 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2401 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2461 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2521 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2581 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2641 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2701 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2761 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2821 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2881 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2941 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3001 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3061 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3121 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3181 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3241 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     3301 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     3361 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     3421 aggaaacagc tatgacatga ttacgaattg gcgcgcctag gccggcc
//
