LOCUS       sequence-207464-        2997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG822
FEATURES             Location/Qualifiers
     source          1..2997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(325..327,328..330,331..1041)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(370..391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(631..650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     978..999
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1042..1062
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1111..1129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1197..1301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1523..2314
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2485..2997,1..76)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2974..2993
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatggtg agcaagggcg aggagctgtt caccggggtg
      361 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      421 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      481 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gaagtgcttc
      541 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      601 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      661 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      721 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      781 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      841 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      901 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc
      961 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactcac
     1021 ggcatggacg agctgtacaa gcctaagaag aagagaaagg ttggaggata agctttgaga
     1081 ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta
     1141 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1201 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1261 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt
     1321 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     1381 atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     1441 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     1501 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     1561 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1621 gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac
     1681 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1741 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1801 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     1861 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     1921 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     1981 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     2041 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     2101 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     2161 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     2221 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt
     2281 aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat
     2341 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2401 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2461 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2521 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2581 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2641 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2701 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2761 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2821 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2881 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2941 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatag
//
