LOCUS       sequence-207465-        4251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG587
FEATURES             Location/Qualifiers
     source          1..4251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     336..353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2471..2489)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2557..2661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2883..3674
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3845..4251,1..182)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
       61 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      241 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      301 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      361 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct
      421 gtggtctcaa ggtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
      481 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
      541 cgcgttacaa gaaagccgtg caattgctgt gccaggcagt tttaacgatc agttcgccga
      601 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttatacctaa
      661 aggtaagtct tactctctct tttttggtct gtatttttaa ttttttgaag tatactattt
      721 gtactgacgc taataatctt ttttcaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      781 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      841 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      901 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      961 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
     1021 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
     1081 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtacttcatg
     1141 cttcaacgtg taacttaaga gatactgtgt gaaattttat atttccatac atttgcttga
     1201 cctttgcttt ttgtcaattt ttttcccctt acaggtggtg gccaatggtg atgtcagcgt
     1261 tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt
     1321 gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt
     1381 cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt
     1441 ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg
     1501 tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga
     1561 ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc
     1621 tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc
     1681 tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact
     1741 gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga
     1801 gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc
     1861 ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa
     1921 actcgacccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga
     1981 taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca
     2041 aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga
     2101 gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca
     2161 ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca
     2221 ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt
     2281 tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga
     2341 ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg
     2401 tgaaaaaccg cagcagggag gcaaacaatg agctttgaga ccacgaagtg gctcttcagt
     2461 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2521 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2581 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2641 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     2701 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg
     2761 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2821 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2881 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2941 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3001 gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3061 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3121 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3181 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3241 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3301 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     3361 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     3421 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     3481 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     3541 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     3601 cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg
     3661 tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3961 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc a
//
