LOCUS       sequence-207466-       11049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for
            MosSCI in nematode. mCherry is optimized by removing all piRNA 
            targeting sites to allow germline expression. RhoA biosenser..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Zhang D, Tu S, Stubna M, Wu WS, Huang WC, Weng Z, Lee HC
  TITLE     The piRNA targeting rules and the resistance to piRNA silencing in 
            endogenous genes.
  JOURNAL   Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840.
            Epub 2018 Feb 1.
  PUBMED    29420292
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aao2840"; journalName: "Science"; date: "2018-02-2-
            2"; volume: "359"; issue: "6375"; pages: "587-592"
COMMENT     Sequence Label: pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
FEATURES             Location/Qualifiers
     source          1..11049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1120..1144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3589..3609)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3617..3634)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3617..3633)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5460..5482
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5520..5538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5606..5710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5711..5779,5780..6571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5929..5948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6742..7330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7231..7250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8966..8977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(11043..11049,1..14)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtatagaaaa gttgaccttt aaataaaatc gagaaaaaat ggcgtcaaaa gacatatgta
       61 aaagctgttt ctctttgtat attcacacag aatatagccc gattttggag gtgaaaaacc
      121 ggtttttttg tattctttac gccacataaa gtgatatgga agagaaaatc gacatattta
      181 tgtacttgga gtacgagtac attggagaaa aagggtgaac tactggaaaa tctgggaaat
      241 ttcagaaaaa aattccgaaa aatctttttt gctgaaaaaa aaatttgttc gaaatttggg
      301 aaatactgga aaattttcat caaaattaaa gcgattttta acgtttttgc ttgggagata
      361 ctatttgtaa ttttaatgtt tggtgaggac aacagaaatg accttcagcg tacggaaaac
      421 gattaaaaga cacatttgaa tcgaaaatag cgtgaaaaac atgaaaatat cggaaaaata
      481 gctttaaaat tggatttgaa agcaaaaaat gtatcaaaaa tcgagaaaaa tgcttgaaaa
      541 agttgaaaaa ctcaaacaaa aaatcaaaaa aggttttttt aaaaatctgt gagtatcgca
      601 acgaaagcac aacttgaggc atgcgccttt aaaccaaccg taaccaaatt cggaggcaaa
      661 aaaagatttc tcgctgtttt ttcagtttta aaatcacttt tcacgtttct tttcgcagta
      721 attcgtttgc aaaacatctg aaaaaatttt attttataag aaattatata aaaatcgcta
      781 taaattgagt ttttgccccc aaattcagca cggagcactt ctcaattaaa tataatttta
      841 ttttttaaca ttgaaagctt tcataaaatt gagctttgtt tgtaaataat cagtgaaaaa
      901 acacagaaat cttcaaaaaa taaagaaaaa gtttttaaaa atctgtgagt cccgcaacga
      961 aaataaaact ttaggcatgc gcctttaaac caaccgtaac caaattcggc ggcaaaaatg
     1021 gatttctcgc cgttttttca gttttacaat cactttttag gtttcttttt gcagtatttc
     1081 gtttcccaaa caattaaaaa tcaaattttc ttttccagat caagtttgta caaaaaagcc
     1141 ggcctcaaaa tggtctccaa gggagaggaa gataacatgg ctatcatcaa ggagttcatg
     1201 agattcaagg tccacatgga gggatccgtc aacggacacg agttcgagat cgagggagag
     1261 ggagagggaa gaccatacga gggaacccag accgctaagc ttaaggtcac caagggagga
     1321 ccagtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagcttcca
     1381 ttcgcttggg atatcctttc cccacagttc atgtacggat ccaaggccta cgtgaagcac
     1441 ccagctgata tcccagatta ccttaagctc tccttcccag agggattcaa gtgggagaga
     1501 gtgatgaact tcgaggatgg aggagtcgtc accgtcaccc aggattcctc cgtaagttta
     1561 aacagttcgg tactaactaa ccatacatat ttaaattttc agcttcagga tggagagttc
     1621 atctacaagg tcaagcttag aggaacaaac ttcccatccg atggaccagt catgcagaag
     1681 aagaccatgg gatgggaggc ttcctccgag agaatgtacc cagaagatgg agctctcaag
     1741 ggagagatca aacaaagact taaacttaaa gatggagtaa gtttaaacat gattttacta
     1801 actaactaat ctgatttaaa ttttcaggga cactacgatg ctgaggtcaa gaccacctac
     1861 aaggctaaga agcctgtgca gcttccagga gcttacaacg tcaacatcaa gcttgatatc
     1921 acctcccaca acgaggatta caccatcgtc gagcagtacg agagagctga gggaagacac
     1981 tccaccggag gaatggatga gctttacaag ggaggccgcg atgacgatgc aacaacacaa
     2041 tttcctgtac ctgaaagatc acgaaaatca agttctcaac tggcctactc tgtttcgttt
     2101 tatcgaaaaa ttcaacgaga cagaaacgaa gaatcgtcga cagtgctggc cgggccagtt
     2161 atcagtcaaa tcagtccatc tgctccgccg atgtcatcat cgctcacatc acaacagaaa
     2221 cttcgacagc tcaccaccgg gcctgcgaat ggagcacgaa ttgtcgagtc ggctaaggat
     2281 gctcacgatc gtatcaagcg agcgattcaa gttgaagagc agcttgttgc tcaatccaag
     2341 agagcaatga tattggcccg ggataagcca agtttccgtg gatcgcgtga ggaatttgaa
     2401 gcacaatggg caatgcttcg tcatgtggaa aagcatcgag cacttctcac tgagtatgat
     2461 cgattgaaac gagatggacc gagaattatc gatggaccac gtggaacgat aacagtatcc
     2521 cagttgtcag tgaacatggc tcgtgattat gtgtcagcta atattgcgtc aagcaagaaa
     2581 tccgatgaag tcttctattt cgcggcgatt ctccgctacg gcgagcaagt cgacgtgtcg
     2641 aaaatggtga caagcgacgg tggtctgaat cgtcgtggtg ttctcgagtt tcccgtacca
     2701 ctgatgttga ccgggattcc accggatttc cgagcaactg tcgagattta tggacaacga
     2761 tcgatgcgag aatcgacgtc gcacgaggat aagtacaagt tgaagaattc gacgtttaag
     2821 gcgaagactc gtaacacatt cctgggcggc ggatcaactt catcagcaaa ccagagccta
     2881 ttcgtcgatc cagcagcatc gtcatcatca acttcgtcga ccacatccaa cttcaatttg
     2941 ctcggaacat tctcgttcga catcaattgc cctggaaaac atttgtacaa catgtcacat
     3001 actgtatatc cgctcgaagg tattacacag atgaaagtac gcaaacaggc gatcgatgga
     3061 gctgatatca cttatcacgg attcttgtca atgtatcaga gaactggcga ggggctcgga
     3121 tcgtggacga gatactggtg tgctcttgag aatggagaga tgaagttttg gaagcagcca
     3181 gaagatgagg gaactaaggg ttacactgca ctaatggatc tttcaacgtg ttgccgttcc
     3241 gaaggtgcat cggtagttga agacatttgc ccattcccga atagcttcca cattgatgtt
     3301 tgggctccaa aaatggatac atcggatcca cgtggcattg agcgtcttcg agttatgctg
     3361 gcggccgata cagctcaaga tcttcaaaca tggctgtcac tgatcaattc tacgtcgaag
     3421 cagttgtgca cgtggagaaa tccgattgtt aatcagtagg ctagcgataa ttttgccgta
     3481 ttttccatat tttgttttgt atatttatcc actcaccccc tctctttgtc ctgtgaatga
     3541 acttgtgcca aaaaagccaa aaataattta ctttttttaa agacatatca actttattat
     3601 acatagttga taattcactg gccgtcgttt tacactcgag acgtacggtg cgcgcgatgc
     3661 attcgaagat ctgcccacta gtgagtcgta ttataagtgc aagtaagatc agtgtttgtt
     3721 tcgatatcag tctgtttcgt aacggtcttc tgtataacta caaaaaagaa taaaaaaccg
     3781 tatcacaact gagaaaaaag acaaaactta ttgaacagtg ttcctttcaa aaaattattt
     3841 tgtaaaaaat cgagtttttc tccaaaataa cgcacgcaat caaaaacaag aaaactaacg
     3901 tgttctcctg ggcgtggaac tccttatcaa gattaccatt attgtctgtc agaattgata
     3961 tttcaattat ttcatatcta gacaaacccg ggtgtcttgc atttattagt actgtgagtt
     4021 tacatacaaa cgaggcttac tcagataaca tagagtcacc tctaattagc aaaaacccat
     4081 ttctaattag ctatttcgac cagttctaaa gtcaccgtaa ttagaaatgg tttttgtagc
     4141 ttttctaatt agaaatgacc ccgagataag tgaaatatgg cattttctgg gttttctagt
     4201 aagaaatgat cccgcacgta gtgattccgc gttacaagcc aaattcaaat taaaaaagta
     4261 tattacacaa ttcaagttac attattttca ggaaacaatg gcgaagagca aagcagagat
     4321 tcaaaaggag gaaagagaca acagatatga acgaagactt cttgaaaaga gaaagagaca
     4381 tgaggtgagt aaccatgata ataaataatc aagtaagggc tatagattta taaattctgg
     4441 agttaacagt ttcgaaaaaa agtagttttc atatgtgcga aggaatatca aaaatagggg
     4501 aatagcctaa acgagtgaga aaattaaatg tttgttattt tttcaaaaat gtctgcaggg
     4561 ccgttatcct ttcaaatgca ctctatcaaa catagatcat atccatcata tcttatccag
     4621 aatttatatc atatccaaga ttgagagcgt ttcaaggaaa atctcaaaca accatacaat
     4681 tttgtagatc gacaacaagg cctccacttc ttcatcgatg gcctctgcag cacctgcacc
     4741 accaagtttg tatttttcat attaatataa agtgagccac gcatagtcgt ttttaggatt
     4801 tttgccagat cgatgtcgcg caaacagact gttttttttt ctttgaaagc cgtgcgtgac
     4861 atgggccacg gattgtaacg ccgcgctgga tggataaaaa tttattcagt tttttagcct
     4921 ttacaaaata tggttcgact actttcttta tttttacatt tttataggtg ctcattgagc
     4981 tgagatattt tgggtataat cggaaaatcg ctgattttgg tcatttttct gcactaaaga
     5041 ttacacaaaa agttcagtat ttagattcat tgttgaaaat tggggatgat ctacagatac
     5101 atatcgccgt gttcaaaaac acacgtgctg tgacgtcgtg actggggaaa accctggcgt
     5161 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5221 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     5281 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     5341 tgcaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5401 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5461 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     5521 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5581 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5641 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5701 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5761 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5821 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5881 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5941 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6001 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6061 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6121 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6181 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6241 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6301 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6361 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6421 gcgtgggtct cgcggtatca ttgcagcact ggggtcagat ggtaagccct cccgtatcgt
     6481 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6541 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6601 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6661 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7141 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt
     7381 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7441 tgagtgagct gataccagcg gataacaatt tcacagggcc ctgtcggatt atgggctctt
     7501 cttttatcct ctactttctt tctagaaaat gcaaatgttg agtcagaaat tgctgaatgg
     7561 agtacaactg caatgggttt agcttatgtt tctggaacaa ttactggtgg aattgtgatt
     7621 gaaaagtaag gttatatttc atttcaataa atgttaccac agtataacaa aaacaagtta
     7681 cattgttcag agtatgtctg aatgtattat tattattgta tttgtgcatc agtactgtag
     7741 ttgtactgaa gaagggttta aaataatctc aacgttttta agacgagtta gtgagaatac
     7801 ggtcgcagtt aggatgtttg gacatagttt tattagataa tcgtgaacta actcactata
     7861 aaaacccgct aactcattga ataagatagt tttaaacgtt caaaatttga agacaaaatt
     7921 gtatgaataa gtttacagag ttggtcgacg caagcttctc cttctcttca catttctcaa
     7981 taatttagct ctaattgcat tcgtattctt cgcaaaaata cgtattctga ttgacccgat
     8041 gaaatacgga tgcctagttg cattgatcat ctacggttat acttatgggt gaggtttttc
     8101 aaatatattt tgatttaatc gaataatgaa atttcagaac tggagttggt ccaatttcgt
     8161 ggtttatatc ctctgaactg gtacctcaaa aacatcgtag tattgctcaa tctgtggcat
     8221 attcaatgaa tactgtaatg gtggttatca gtacatttac agtattacct ctatattccg
     8281 ttattggtag ttatgctttc cttattctat attcaattcc ttcatttata agtatgctta
     8341 tattatttcg ctacttaccg gaaaccaaag gacgagagat tcatgatatt gtgaatgaac
     8401 ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc gaaattttac ttgatcgagc
     8461 caattgaact tttgttaaga cttacagaaa agtgagagat tttgttacaa aaaagtgaga
     8521 aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc cgaagtatca tataaaaatt
     8581 aaaaagattg tttgacctgg cggaactttt ttgcaaaaac tctgaatatc catggcacat
     8641 gcttcgtgca aaacaggatc caaatgaata tcaacaagag attcttgaag acgacgagcc
     8701 acttgctcag agcattgctt atctcgaatg agacccttgt taaaatcatt tttcaaacat
     8761 tatatgacat tgtcgaaatg tcctcctgat tccatgatgg tagcaaactc acttcgtctt
     8821 tgagccaatt tatccaagtc cttgtaaaaa gtattcgaaa attgttaacg gataaaatgt
     8881 ttattataat atcaaaaaca atttgtcagt tgaccacttt ttgatatatt tgacagaaac
     8941 cgggatgaat tggctcaaag ttagggcgcc cttctattac agggtttctg ataacaaacc
     9001 ggttattaac tcccaacaag ggatgatttc aattcatcat gctcaaattg acccaaatta
     9061 agttacatga caaattcatc gcccttttca actcttcttg gctcatcatc tgttattctg
     9121 ttcctattct gtctgcaccc tatacccctt tgcatactct ctcgtcattc ctttcttgga
     9181 tagtgtcttc ttctccccag ctctgctact tctatgactt gcgcccgctg cttttccgct
     9241 cgttcctctc ctctctgacg tcttcttctg cttcttttct ccttcctttt tcccttctca
     9301 atcttcctct ttttcccatt tctgtcaatc attcgaagaa gaagaagaag aagaccctca
     9361 ttcatttcat ttttttttct gtcggtgtgt gctgctcggt taagagtgag ctctctattc
     9421 cacgtcttct tctttttctt cttcgattcg aatcaatcac tccaccaaaa cgcattcgtt
     9481 tttgggattc accccgcggt tcgcaatagg tttctttttc aaattattag cgttataaat
     9541 agaaaaatgg tggagtttca ataaaaatga taatttacaa agtgtatttt gattacatgt
     9601 actcaaaaag gttgaaattt ttcataccag ttttccggaa atccatctga tatcattatc
     9661 gtatttttct ttttaaaaat gttttcaaaa aaaaacaaaa tatagctggt tatttggcac
     9721 cctctaatta ccattttcct gtcacaccac actcttttcc ttcttccact tctttccgtt
     9781 ttcagccgct tccaaccaaa ccgatatgaa agccgagcaa caacgatcga ttccacccgg
     9841 ctcggcgacc ttcccgtcgc aggtgagact cagaaaacta gagaaaccgc tcaactaact
     9901 cttgatatcc gatttcattc ttttctcttt tctttttgtt gaacttttcc atatttccag
     9961 atgccacggc caccaccaag caccgaacaa ggaatcacaa cggaatcgga gcttgcgaag
    10021 aaagctcaaa tcactccgaa cgacgtttta gcacttccgg gaatcactca aggtatcttg
    10081 ctccctttgg atttcagttt aaaacataaa tttaggattc ttatgctccc catctgcgaa
    10141 catctataac atcgagttca ccaagttcca aatccgtgat ctggacactg agcaagtgct
    10201 tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac aggagtcggc
    10261 aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac
    10321 tgtggaattc aaagtaggag acatcccaat ccatcatttc cgaatgatcg aacgtcactt
    10381 cttcaaagat cgccttctga agtgttttga ctttgaattc ggattctgta ttccgaattc
    10441 acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac tcagtgagtc
    10501 attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg acatgatcaa
    10561 caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac tcgtcatgca
    10621 caacaaagcc gactactcat atgatgcata aatatttaat acaaaaatgt tctggataat
    10681 tattctgtcg aatagaaaaa aaactccaaa tgtgattaaa ttccaataat tcctgtctag
    10741 tttgttcctt ccccttcccc ttctcatgtt caatgcattc ctaagctttt cagttccccc
    10801 cttgtttcta tattttttcg ctgtcctgtc acactcgcta aaaacactaa tcacacggaa
    10861 atctgttttc aataaaaact ccaactttaa ctcattttca atttcaactg aaagattttt
    10921 tcattagaga atgtctagaa ctaggcccgg gctacgtaat acgactcact taaggccttg
    10981 actagagggt accagagctc acctaggcag gaacagctat gaccatgatt acgccaagct
    11041 atcaacttt
//
