LOCUS       sequence-207468-        1387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in mammalian cells of of full-length SPRTN protein 
            carrying E112A mutation, a catalytically-dead mutant, and Flag-tag 
            at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Lessel D, Vaz B, Halder S, Lockhart PJ, Marinovic-Terzic I, 
            Lopez-Mosqueda J, Philipp M, Sim JC, Smith KR, Oehler J, Cabrera E, 
            Freire R, Pope K, Nahid A, Norris F, Leventer RJ, Delatycki MB, 
            Barbi G, von Ameln S, Hogel J, Degoricija M, Fertig R, Burkhalter 
            MD, Hofmann K, Thiele H, Altmuller J, Nurnberg G, Nurnberg P, Bahlo 
            M, Martin GM, Aalfs CM, Oshima J, Terzic J, Amor DJ, Dikic I, 
            Ramadan K, Kubisch C
  TITLE     Mutations in SPRTN cause early onset hepatocellular carcinoma, 
            genomic instability and progeroid features.
  JOURNAL   Nat Genet. 2014 Nov;46(11):1239-44. doi: 10.1038/ng.3103. Epub 2014 
            Sep 28.
  PUBMED    25261934
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3103"; 
            journalName: "Nat Genet"; date: "2014-11"; volume: "46"; issue: 
            "11"; pages: "1239-44"
COMMENT     Sequence Label: pCDNA3-Flag-SPRTN-E112A
FEATURES             Location/Qualifiers
     source          1..1387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..57
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             1351..1364
                     /estimated_length=14
     gap             1369..1382
                     /estimated_length=14
ORIGIN
        1 tnnnnttagc ttggtaccga gctcggatcc ntggattata aggatgacga tgataaaatg
       61 gatgatgact tgatgttggc actgcggctt caggaggagt ggaacttgca ggaggcggag
      121 cgcgatcatg cccaggagtc cctgtcgcta gtggacgcgt cgtgggagtt ggtggacccc
      181 acaccggact tgcaggcact gtttgttcag tttaacgacc aattcttctg gggccagctg
      241 gaggccgtcg aggtgaagtg gagcgtgcga atgaccctgt gtgctgggat atgcagctat
      301 gaagggaagg gtggaatgtg ttccatccgt ctcagcgaac cccttttgaa gttgaggcca
      361 agaaaggatc ttgtagagac cctcctgcat gcaatgatac atgcctattt atttgtcact
      421 aataacgaca aagaccgaga agggcatggt ccagaatttt gtaaacatat gcatcgcatc
      481 aacagcctga ctggagccaa tataacggta taccatactt ttcacgatga ggtggatgag
      541 tatcggcgac actggtggcg ctgcaatggg ccgtgccagc acaggccacc gtattacggc
      601 tatgtcaaac gagctactaa cagggaaccc tctgctcatg actattggtg ggctgagcac
      661 cagaaaacct gtggaggcac ttacataaaa atcaaggaac cagagaatta ctcaaaaaaa
      721 ggcaaaggaa aggcaaaact aggaaaggaa ccagtattgg ccgcagagaa taaagataaa
      781 cccaacagag gtgaggccca gctagtaatc ccttttagtg ggaaaggata tgttctagga
      841 gaaacaagca atttaccttc acctgggaaa ctgatcactt cacatgccat taataaaacc
      901 caagatcttt taaatcaaaa ccattcagca aatgctgtaa gacttaattc taaaatcaag
      961 gtgaaatttg aacagaatgg ntcaagtaaa aattctcatc tggtctcccc tgctgttagt
     1021 aacagtcacc aaaatgttct aagcaactac tttcctagag tatcatttgc caaccaaaag
     1081 gctttcanag gtgtgaatgg atctncanga tagtgtacag ttggcanatc cctaaaactc
     1141 agtctctcta gtctcanana agggtttcat ctnctanata tccctannaa attcttcaaa
     1201 gtaacggaat cagcatctgt gatgccatcc cnggangnnn anngggnnnt nangannnnt
     1261 tccnaataan gacctaggct annaannnnn annactnttt tgacantttt ttnncannaa
     1321 ngancaaatn aaannnnnnn nnaannatcc nnnnnnnnnn nnnnanccnn nnnnnnnnnn
     1381 nnaantc
//
