LOCUS       sequence-207469-        1353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in mammalian cells of of full-length SPRTN protein 
            carrying Y117C mutation, a catalytically-impaired mutant, and 
            Flag-tag at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Vaz B, Popovic M, Newman JA, Fielden J, Aitkenhead H, Halder S, 
            Singh AN, Vendrell I, Fischer R, Torrecilla I, Drobnitzky N, Freire 
            R, Amor DJ, Lockhart PJ, Kessler BM, McKenna GW, Gileadi O, Ramadan 
            K
  TITLE     Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled 
            DNA-Protein Crosslink Repair.
  JOURNAL   Mol Cell. 2016 Nov 17;64(4):704-719. doi: 
            10.1016/j.molcel.2016.09.032. Epub 2016 Oct 27.
  PUBMED    27871366
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.032"; journalName: "Mol Cell"; date: 
            "2016-11-17- 17"; volume: "64"; issue: "4"; pages: "704-719"
COMMENT     Sequence Label: pCDNA3-Flag-SPRTN-Y117C
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..56
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tnanttagct tggtaccgag ctcggatcca tggattataa ggatgacgat gataaaatgg
       61 atgatgactt gatgttggca ctgcggcttc aggaggagtg gaacttgcag gaggcggagc
      121 gcgatcatgc ccaggagtcc ctgtcgctag tggacgcgtc gtgggagttg gtggacccca
      181 caccggactt gcaggcactg tttgttcagt ttaacgacca attcttctgg ggccagctgg
      241 aggccgtcga ggtgaagtgg agcgtgcgaa tgaccctgtg tgctgggata tgcagctatg
      301 aagggaaggg tggaatgtgt tccatccgtc tcagcgaacc ccttttgaag ttgaggccaa
      361 gaaaggatct tgtagagacc ctcctgcatg aaatgataca tgcctgttta tttgtcacta
      421 ataacgacaa agaccgagaa gggcatggtc cagaattttg taaacatatg catcgcatca
      481 acagcctgac tggagccaat ataacggtat accatacttt tcacgatgag gtggatgagt
      541 atcggcgaca ctggtggcgc tgcaatgggc cgtgccagca caggccaccg tattacggct
      601 atgtcaaacg agctactaac agggaaccct ctgctcatga ctattggtgg gctgagcacc
      661 agaaaacctg tggaggcact tacataaaaa tcaaggaacc agagaattac tcaaaaaaag
      721 gcaaaggaaa ggcaaaacta ggaaaggaac cagtattggc cgcagagaat aaagataaac
      781 ccaacagagg tgaggcccag ctagtaatcc cttttagtgg gaaaggatat gttctaggag
      841 aaacaagcaa tttaccttca cctgggaaac tgatcacttc acatgccatt aataaaaccc
      901 aagatctttt aaatcaaaac cattcagcaa atgctgtaag acttaattct aaaatcaagg
      961 tgaaatttga acagaatggt tcaagtaaaa attctcatct ggtctcccct gctgttagta
     1021 acagtcacca aaatgttcta agcaactact ttcctagagt atcatttgcc aaccaaaagg
     1081 ctttcanagn tgtgaatgga tctncaagga tagtgtacag ttggcaacat ccctaaaact
     1141 cagtcnnttc tagttctcan anaaggnttc atcttnctan atatccctaa naaattcttc
     1201 aaagtaacgg aatcagcatc tgngatgcca tcccaggatn nnnagtgggn nnngaagana
     1261 cnttncnnnn aannacctnn ntagaaanan nnannnctgn ttttgannna tttttttnnn
     1321 nnnaaannnn ncnaaataaa aaagnnnngn naa
//
