LOCUS       sequence-20747-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GAL-ccdB-TAP
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(30..154)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(162..178)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(162..178)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(193..634)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(199..222)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    517..634
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(658..678)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(658..676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaagcctggg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt
       61 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
      121 acgtttctcg ttcagctttt ttgtacaaac ttgtgatggg ggatccacta gttctagaat
      181 ccggggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata
      241 cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt attagttaaa
      301 gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca tcgcttcgct
      361 gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta
      421 atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat
      481 aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca
      541 tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccttcg
      601 gagggctgtc acccgctcgg cggcttctaa tccgtactag agctccagct tttgttccct
      661 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca t
//
