LOCUS       sequence-207471-        2043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in E. coli of full-length wild-type SPRTN protein, with 
            His and ZB tags at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Vaz B, Popovic M, Newman JA, Fielden J, Aitkenhead H, Halder S, 
            Singh AN, Vendrell I, Fischer R, Torrecilla I, Drobnitzky N, Freire 
            R, Amor DJ, Lockhart PJ, Kessler BM, McKenna GW, Gileadi O, Ramadan 
            K
  TITLE     Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled 
            DNA-Protein Crosslink Repair.
  JOURNAL   Mol Cell. 2016 Nov 17;64(4):704-719. doi: 
            10.1016/j.molcel.2016.09.032. Epub 2016 Oct 27.
  PUBMED    27871366
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.032"; journalName: "Mol Cell"; date: 
            "2016-11-17- 17"; volume: "64"; issue: "4"; pages: "704-719"
COMMENT     Sequence Label: pNIC-ZB-SPRTNwt-FL
FEATURES             Location/Qualifiers
     source          1..2043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     gap             1477..1491
                     /estimated_length=15
     gap             1614..1629
                     /estimated_length=16
     gap             1648..1657
                     /estimated_length=10
     gap             1820..1833
                     /estimated_length=14
     gap             1917..1930
                     /estimated_length=14
     gap             1983..1996
                     /estimated_length=14
     gap             1999..2017
                     /estimated_length=19
ORIGIN
        1 tcnngctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc
       61 aagcttgtcg acggagctcg aattcggatc cgtatccacc tttactgtca aagactttct
      121 tcgcttttga ctttgatgct gtcaccttca aggcaccagt ccaagtgctc attaatctga
      181 gactccagaa cttcattctg acaaactggg caattaacca ttttgctctg actggatgaa
      241 ctgctggaat tctgagctgt ggttgtacta tactttggat cattaccact gctttttatt
      301 tgctctttct tgataaaaaa attgtcaaaa acagtcttat cttctagcct aggtcgttta
      361 tttgggaatg tatcttcaga cccactcaca tcctgggatg gcatcacaga tgctgattcc
      421 gttacttttg aagaatttct tagggatatc ttagaagatg aaacccttct ctgagaacta
      481 gaagagactg agtttttagg gatgttgcca actgttacac ttatccttgg agatccattc
      541 acacctctga aagccttttg gttggcaaat gatactctag gaaagtagtt gcttagaaca
      601 ttttggtgac tgttactaac agcaggggag accagatgag aatttttact tgaaccattc
      661 tgttcaaatt tcaccttgat tttagaatta agtcttacag catttgctga atggttttga
      721 tttaaaagat cttgggtttt attaatggca tgtgaagtga tcagtttccc aggtgaaggt
      781 aaattgcttg tttctcctag aacatatcct ttcccactaa aagggattac tagctgggcc
      841 tcacctctgt tgggtttatc tttattctct gcggccaata ctggttcctt tcctagtttt
      901 gcctttcctt tgcctttttt tgagtaattc tctggttcct tgatttttat gtaagtgcct
      961 ccacaggttt tctggtgctc agcccaccaa tagtcatgag cagagggttc cctgttagta
     1021 gctcgtttga catagccgta atacggtggc ctgtgctggc acggcccatt gcagcgccac
     1081 cagtgtcgcc gatactcatc cacctcatcg tgaaaagtat ggtataccgt tatattggct
     1141 ccagtcaggc tgttgatgcg atgcatatgt ttacaaaatt ctggaccatg cccttctcgg
     1201 tctttgtcgt tattantgac aaataaatag gcatgtatca tttcatgcag gaggntctct
     1261 acaanntcct ttcttggcct caacttcaaa aggggttcgc tnaancggat ggaanncatt
     1321 ccacccttcc cttcatagct gcanatccca gnnnanangg ntcattcnna nnnnccactt
     1381 caccnnnacg gcctccantn ggccccaaaa aanttggncn ttaaannnaa aaaannnncn
     1441 ngncaanncc ggggggggnt ccannaatnc ccnnnannnn nnnnnnnnnn naagggnnnn
     1501 nnngggnnnn nnnnccgnnn cccnnnnnng naanttcnnn ncnnnntnnn ngccnnnngn
     1561 cnnntnaann tnnnnntccn nggnttngga aannnnngtn nnnnngnaac cntnnnnnnn
     1621 nnnnnnnnnt nnaanttnnn nnnntncnnn nnnnnnntnn nnnnnggnnn ngnnnnntnn
     1681 nnnnngnnnn naaaannnnn gnnnnnnntt ttnccnggnt tnnnnnnncc cnnnannggg
     1741 nannnnncng ggnnnnttnn nnnaccnnnn nnnnnacctn nnnnccccnc cnnaaaaann
     1801 nnnnnnnaaa nggnnnnaan nnnnnnnnnn nnntnnannn naaannaaaa nnttttnnnn
     1861 nggnnnnnnn ttttccnncn annnnnnnan nnnngggntn naatttnnng gnnnaannnn
     1921 nnnnnnnnnn cccggnnnnn ngggnnnnnn nnncnnnccn nngggggnng ggnncnnttn
     1981 ccnnnnnnnn nnnnnnaann nnnnnnnnnn nnnnnnnttt tnnnnngnnn nggncccnnn
     2041 nng
//
