LOCUS       sequence-207472-        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in E. coli of full-length wild-type SPRTN protein, with 
            His and ZB tags at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Vaz B, Popovic M, Newman JA, Fielden J, Aitkenhead H, Halder S, 
            Singh AN, Vendrell I, Fischer R, Torrecilla I, Drobnitzky N, Freire 
            R, Amor DJ, Lockhart PJ, Kessler BM, McKenna GW, Gileadi O, Ramadan 
            K
  TITLE     Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled 
            DNA-Protein Crosslink Repair.
  JOURNAL   Mol Cell. 2016 Nov 17;64(4):704-719. doi: 
            10.1016/j.molcel.2016.09.032. Epub 2016 Oct 27.
  PUBMED    27871366
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.032"; journalName: "Mol Cell"; date: 
            "2016-11-17- 17"; volume: "64"; issue: "4"; pages: "704-719"
COMMENT     Sequence Label: pNIC-ZB-SPRTNwt-FL
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..241
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             248..268
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     gap             1598..1608
                     /estimated_length=11
     gap             1732..1742
                     /estimated_length=11
     gap             1791..1802
                     /estimated_length=12
     gap             1851..1868
                     /estimated_length=18
     gap             1881..1891
                     /estimated_length=11
     gap             1913..1927
                     /estimated_length=15
     gap             1956..1966
                     /estimated_length=11
ORIGIN
        1 ctagaataat tttgtttanc tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtggataaca agttcaacaa ggagcgtcga agagctcgcc gtgaaattcg
      121 ccatctgccg aacctgaacc gcgaacagcg tcgcgcattt attcgcagcc tgcgcgatga
      181 tccgagccag agcgcgaacc tgctggcgga agcgaagaag ctgaacgatg cgcagccgaa
      241 gggtaccgag aacctgtact tccaatccat ggatgatgac ttgatgttgg cactgcggct
      301 tcaggaggag tggaacttgc aggaggcgga gcgcgatcat gcccaggagt ccctgtcgct
      361 agtggacgcg tcgtgggagt tggtggaccc cacaccggac ttgcaggcac tgtttgttca
      421 gtttaacgac caattcttct ggggccagct ggaggccgtc gaggtgaagt ggagcgtgcg
      481 aatgaccctg tgtgctggga tatgcagcta tgaagggaag ggtggaatgt gttccatccg
      541 tctcagcgaa ccccttttga agttgaggcc aagaaaggat cttgtagaga ccctcctgca
      601 tgaaatgata catgcctatt tatttgtcac taataacgac aaagaccgag aagggcatgg
      661 tccagaattt tgtaaacata tgcatcgcat caacagcctg actggagcca atataacggt
      721 ataccatact tttcacgatg aggtggatga gtatcggcga cactggtggc gctgcaatgg
      781 gccgtgccag cacaggccac cgtattacgg ctatgtcaaa cgagctacta acagggaacc
      841 ctctgctcat gactattggt gggctgagca ccagaaaacc tgtggaggca cttacataaa
      901 aatcaaggaa ccagagaatt actcaaaaaa aggcaaagga aaggcaaaac taggaaagga
      961 accagtattg gccgcagaga ataaagataa acccaacaga ggtgaggccc agctagtaat
     1021 cccttttagt gggaaaggat atgttctagg agaaacaagc aatttacctt cacctgggaa
     1081 actgatcact tcacatgcca ttaataaaac ccaagatctt ttaaatcaaa accattcagc
     1141 aaatgctgta agacttaatt ctaaaatcaa ggtgaaattt gaacagaatg gttcaagtaa
     1201 aaattctcat ctggnctccc ctgctgttag taacagtcac caaannnttc taagcaacta
     1261 ctttncctnn agnnncattt tgccaaccaa aaggntttnc aaanggnnng aatggnnnnt
     1321 ccnaaggata annnnaanna gttggncaan nncctnaaaa nntnnnnnnn nttcnagttn
     1381 nnaaaaaang ggtttcannn tnnnaanntn nccntnaaaa nttnnnnnaa annntaacng
     1441 natccnncat nnnggnatgg ccnncccnag gaangnnnnn ngggnnnnna aanntnnntt
     1501 nccaannnaa ancnnacnnn agnncttana anaaaaaann nngnttttnn nnnanntttt
     1561 ttttnncnan naannnncna nnttaaannn nnnnnngnnn nnnnnnnncc aannnnnnnn
     1621 nannnccccn nnnnnnnnan anttnccnng gggnnnnnnn cccnnnncnn nnnnaaaang
     1681 gnnnnnnntn gcccnnnttt tnnnnaaaan nnnnatnnnn nggnnnnnnc cnnnnnnnnn
     1741 nngnnncnnt nnggannnnn ggggcnnntn nnnngggnaa nnnnnnnnna nnnnnnnnnn
     1801 nnaaaaaaan nntttttnan nnnaaaangg ggggnnnnnn ngnnnnnnaa nnnnnnnnnn
     1861 nnnnnnnnaa aaatnnnggg nnnnnnnnnn ntnnnncccc ccccccnnnn ncnnnnnnnn
     1921 nnnnnnnggn nnnnnannnc cnnnnnnnna nnnnannnnn nnnnnncnnn cnnnnnnaan
     1981 naaannaann ncnnnnnngg nnnnnnnnaa nnnnnnnnng gnnntttttc naaa
//
