LOCUS       sequence-207473-        8031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible T7*RNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Boehm CR, Grant PK, Haseloff J
  TITLE     Programmed hierarchical patterning of bacterial populations.
  JOURNAL   Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3.
  PUBMED    29472537
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-22"; volume: "9(1):776. doi"; pages: " 
            10.1038/s41467-018-03069-3"
COMMENT     Sequence Label: pTara:500*
FEATURES             Location/Qualifiers
     source          1..8031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1289
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1212..1231
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(4065..4082)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4235..4321
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4413..4440
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4459..4550
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5008..5463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5095..5114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5305..5326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6019..6678)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6593..6612
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6679..6781)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7173..7190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7307..7852)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7387..7406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7928..7947
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtcctaggac attgattatt tgcacggcgt
     1201 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
     1261 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagcg agctctagaa
     1321 agacttactt aataacattc tttttctaat atgaacacga ttaacatcgc taagaacgac
     1381 ttctctgaca tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag
     1441 cgtttagctc gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc
     1501 ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc
     1561 aagcctctca tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag
     1621 gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc agttcctgca agaaatcaag
     1681 ccggaagccg tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac
     1741 aatacaaccg ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc
     1801 ttcggtcgta tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc
     1861 aacaagcgcg tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg
     1921 ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt
     1981 catgtaggag tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac
     2041 cgccaaaatg ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac
     2101 gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct
     2161 tgcgtagttc ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt
     2221 cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac
     2281 gtttacatgc ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc
     2341 aacaagaaag tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag
     2401 gacatccctg cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat
     2461 cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct
     2521 cgcaagtctc gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac
     2581 cataaggcca tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca
     2641 atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa
     2701 ccaatcggta aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc
     2761 gataaggttc cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg
     2821 gcttgcgcta agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc
     2881 ttccttgcgt tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc
     2941 tcccttccgc tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc
     3001 cgagatgagg taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc
     3061 tacgggattg ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc
     3121 gataacgaag tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag
     3181 ctgggcacta aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact
     3241 aagagttcag tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg
     3301 ctggaagata ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg
     3361 aatcaggctg ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta
     3421 gctgcggttg aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc
     3481 aaagataaga agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct
     3541 gatggtttcc ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg
     3601 ttcctcggtc agttccgctt acagcctacc attaacacca acaaagatag cgagattgat
     3661 gcacacaaac aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac
     3721 cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt
     3781 cacgactcct tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa
     3841 actatggttg acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct
     3901 gaccagttgc acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg
     3961 aacctccgtg acatcttaga gtcggacttc gcgttcgcgt aatctagagt cgacctgcag
     4021 gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     4081 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     4141 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     4201 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     4261 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     4321 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     4381 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     4441 tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4501 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4561 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4621 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca gcaaactatt
     4681 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4741 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4801 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4861 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4921 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4981 ttactcatat atactttaga ttgatttacg cgccctgtag cggcgcatta agcgcggcgg
     5041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5221 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5341 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtcag gcatttgaga
     5821 agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag
     5881 cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct
     5941 gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca
     6001 ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     6061 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     6121 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     6181 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     6241 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     6301 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     6361 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     6421 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat
     6481 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     6541 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     6601 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     6661 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     6721 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     6781 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     6841 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
     6901 gggtgatgct gccaacttac tgatttagtg tattatggtg tttttgaggt gctccagtgg
     6961 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
     7021 aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga
     7081 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
     7141 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
     7201 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
     7261 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
     7321 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
     7381 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
     7441 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
     7501 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     7561 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
     7621 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
     7681 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
     7741 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
     7801 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
     7861 tcttattaat cagataaaat atttctaggc tcatgagccc gaagtggcga gcccgatctt
     7921 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     7981 cggccacgat gcgtccggcg tagaggatct gctcatgttt gacagcttat c
//
