LOCUS       sequence-207478-        2900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Mbefo MK, Fares MB, Paleologou K, Oueslati A, Yin G, Tenreiro S, 
            Pinto M, Outeiro T, Zweckstetter M, Masliah E, Lashuel HA
  TITLE     Parkinson disease mutant E46K enhances alpha-synuclein 
            phosphorylation in mammalian cell lines, in yeast, and in vivo.
  JOURNAL   J Biol Chem. 2015 Apr 10;290(15):9412-27. doi: 
            10.1074/jbc.M114.610774. Epub 2015 Feb 5.
  PUBMED    25657004
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.610774"; journalName: "J Biol Chem"; date: 
            "2015-04-10- 10"; volume: "290"; issue: "15"; pages: "9412-27"
COMMENT     Sequence Label: pT7-7 asyn E46K
FEATURES             Location/Qualifiers
     source          1..2900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      9..597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     498..517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     751..768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1003..1025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1090..1109
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1090..1108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1140..1162
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1729..2520,2521..2589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2352..2371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2590..2694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2762..2780
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
       61 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      121 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
      181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      781 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataggaaga
      841 tcttccggaa gatcttccta tggtgcactc tcagtacaat ctgctctgat gcgctacgtg
      901 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      961 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1021 agaggttttc accgtcatca ccgaaacgcg cgaggcccag cgattcgaac ttctgataga
     1081 cttcgaaatt aatacgactc actataggga gaccacaacg gtttccctct agaaataatt
     1141 ttgtttaact ttaagaagga gatatacata tggatgtatt catgaaagga ctttcaaagg
     1201 ccaaggaggg agttgtggct gctgctgaga aaaccaaaca gggtgtggca gaagcagcag
     1261 gaaagacaaa agagggtgtt ctctatgtag gctccaaaac caagaaggga gtggtgcatg
     1321 gtgtggcaac agtggctgag aagaccaaag agcaagtgac aaatgttgga ggagcagtgg
     1381 tgacgggtgt gacagcagta gcccagaaga cagtggaggg agcagggagc attgcagcag
     1441 ccactggctt tgtcaaaaag gaccagttgg gcaagaatga agaaggagcc ccacaggaag
     1501 gaattctgga agatatgcct gtggatcctg acaatgaggc ttatgaaatg ccttctgagg
     1561 aagggtatca agactacgaa cctgaagcct aagaaatatc tttgctccca gtttcttgag
     1621 atctgctgac agatgttcca tccaagcttg agagcttatc gatgataagc tgtcaaacat
     1681 gagaattaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2341 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2701 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2761 aaataggcgt atcacgaggc cctttcgtct tcaagaataa aaggatctag gtgaagatcc
     2821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2881 accccgtaga aaagatcaaa
//
