LOCUS       sequence-207480-        2892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Rospigliosi CC, McClendon S, Schmid AW, Ramlall TF, Barre P, Lashuel
            HA, Eliezer D
  TITLE     E46K Parkinson's-linked mutation enhances C-terminal-to-N-terminal 
            contacts in alpha-synuclein.
  JOURNAL   J Mol Biol. 2009 May 22;388(5):1022-32. doi: 
            10.1016/j.jmb.2009.03.065. Epub 2009 Apr 5.
  PUBMED    19345692
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2009.03.065"; journalName: "J Mol Biol"; date: 
            "2009-05-22- 22"; volume: "388"; issue: "5"; pages: "1022-32"
COMMENT     Sequence Label: pT7-7 asyn A30P
FEATURES             Location/Qualifiers
     source          1..2892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      9..597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     498..517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     751..768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1003..1025
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1090..1109
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1090..1108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1140..1162
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1721..2512,2513..2581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2344..2363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2582..2686)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2754..2772
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
       61 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      121 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
      181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      781 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataggaaga
      841 tcttccggaa gatcttccta tggtgcactc tcagtacaat ctgctctgat gcgctacgtg
      901 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      961 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1021 agaggttttc accgtcatca ccgaaacgcg cgaggcccag cgattcgaac ttctgataga
     1081 cttcgaaatt aatacgactc actataggga gaccacaacg gtttccctct agaaataatt
     1141 ttgtttaact ttaagaagga gatatacata tggatgtatt catgaaagga ctttcaaagg
     1201 ccaaggaggg agttgtggct gctgctgaga aaaccaaaca gggtgtggca gaagcaccag
     1261 gaaagacaaa agagggtgtt ctctatgtag gctccaaaac caaggaggga gtggtgcatg
     1321 gtgtggcaac agtggctgag aagaccaaag agcaagtgac aaatgttgga ggagcagtgg
     1381 tgacgggtgt gacagcagta gcccagaaga cagtggaggg agcagggagc attgcagcag
     1441 ccactggctt tgtcaaaaag gaccagttgg gcaagaatga agaaggagcc ccacaggaag
     1501 gaattctgga agatatgcct gtggatcctg acaatgaggc ttatgaaatg ccttctgagg
     1561 aagggtatca agactacgaa cctgaagcct aagaaatatc tttgctccca gtttcttgag
     1621 atctgctgac agatgttcca tccaagctta tcgatgataa gctgtcaaac atgagaatta
     1681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     2341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2701 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     2761 gtatcacgag gccctttcgt cttcaagaat aaaaggatct aggtgaagat cctttttgat
     2821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2881 gaaaagatca aa
//
