LOCUS       sequence-207487-        5746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG545
FEATURES             Location/Qualifiers
     source          1..5746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     230..247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3860..3878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3946..4050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4272..5063
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(5234..5746,1..76)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5723..5742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
       61 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      301 tcattaatca ctctgtggtc tcaaatggat cccattcgtt cgcgcacacc aagtcctgcc
      361 cgcgagcttc tgcccggacc ccaacccgat ggggttcagc cgactgccga tcgtggggtg
      421 tctccgcctg ccggcggccc cctggatggc ttgcccgctc ggcggacgat gtcccggacc
      481 cggctgccat ctccccctgc cccctcacct gcgttctcgg cgggcagctt cagtgacctg
      541 ttacgtcagt tcgatccgtc actttttaat acatcgcttt ttgattcatt gcctcccttc
      601 ggcgctcacc atacagaggc tgccacaggc gagtgggatg aggtgcaatc gggtctgcgg
      661 gcagccgacg cccccccacc caccatgcgc gtggctgtca ctgccgcgcg gccgccgcgc
      721 gccaagccgg cgccgcgacg acgtgctgcg caaccctccg acgcttcgcc ggccgcgcag
      781 gtggatctac gcacgctcgg ctacagccag cagcaacagg agaagatcaa accgaaggtt
      841 cgttcgacag tggcgcagca ccacgaggca ctggtcggcc atgggtttac acacgcgcac
      901 atcgttgcgc tcagccaaca cccggcagcg ttagggaccg tcgctgtcaa gtatcaggac
      961 atgatcgcag cgttgccaga ggcgacacac gaagcgatcg ttggcgtcgg caaacagtgg
     1021 tccggcgcac gcgccctgga ggccttgctc acggtggcgg gagagttgag aggtccaccg
     1081 ttacagttgg acacaggcca acttctcaag attgcaaaac gtggcggcgt gaccgcagtg
     1141 gaggcagtgc atgcatggcg caatgcactg acgggtgccc ccctgaacct gaccccggag
     1201 caggtggtgg ccatcgccag ccacgatggc ggcaagcagg cgctggagac ggtgcagcgg
     1261 ctgttgccgg tgctgtgcca ggcccatggc ctgaccccgc agcaggtggt ggccatcgcc
     1321 agcaatggcg gtggcaagca ggcgctggag acggtgcagc ggctgttgcc ggtgctgtgc
     1381 caggcccatg gcctgacccc gcagcaggtg gtggccatcg ccagcaatag cggtggcaag
     1441 caggcgctgg agacggtgca gcggctgttg ccggtgctgt gccaggccca tggcctgacc
     1501 ccggagcagg tggtggccat cgccagcaat ggcggtggca agcaggcgct ggagacggtg
     1561 cagcggctgt tgccggtgct gtgccaggcc catggcctga ccccggagca ggtggtggcc
     1621 atcgccagca atattggtgg caagcaggcg ctggagacgg tgcaggcgct gttgccggtg
     1681 ctgtgccagg cccatggcct gaccccggag caggtggtgg ccatcgccag caatattggt
     1741 ggcaagcagg cgctggagac ggtgcaggcg ctgttgccgg tgctgtgcca ggcccatggc
     1801 ctgaccccgg agcaggtggt ggccatcgcc agcaatattg gtggcaagca ggcgctggag
     1861 acggtgcagg cgctgttgcc ggtgctgtgc caggcccatg gcctgacccc ggagcaggtg
     1921 gtggccatcg ccagccacga tggcggcaag caggcgctgg agacggtgca gcggctgttg
     1981 ccggtgctgt gccaggccca tggcctgacc ccggagcagg tggtggccat cgccagccac
     2041 gatggcggca agcaggcgct ggagacggtg cagcggctgt tgccggtgct gtgccaggcc
     2101 catggcctga ccccgcagca ggtggtggcc atcgccagca atggcggtgg caagcaggcg
     2161 ctggagacgg tgcagcggct gttgccggtg ctgtgccagg cccatggcct gaccccggag
     2221 caggtggtgg ccatcgccag caatagcggt ggcaagcagg cgctggagac ggtgcaggcg
     2281 ctgttgccgg tgctgtgcca ggcccatggc ctgaccccgg agcaggtggt ggccatcgcc
     2341 agcaatagcg gtggcaagca ggcgctggag acggtgcagc ggctgttgcc ggtgctgtgc
     2401 caggcccatg gcctgacccc ggagcaggtg gtggccatcg ccagccacga tggcggcaag
     2461 caggcgctgg agacggtgca gcggctgttg ccggtgctgt gccaggccca tggcctgacc
     2521 ccggagcagg tggtggccat cgccagccac gatggcggca agcaggcgct ggagacggtg
     2581 cagcggctgt tgccggtgct gtgccaggcc catggcctga ccccggagca ggtggtggcc
     2641 atcgccagcc acgatggcgg caagcaggcg ctggagacgg tgcagcggct gttgccggtg
     2701 ctgtgccagg cccatggcct gaccccgcag caggtggtgg ccatcgccag caatggcggc
     2761 ggcaggccgg cgctggagac ggtgcagcgg ctgttgccgg tgctgtgcca ggcccatggc
     2821 ctgaccccgg agcaggtggt ggccatcgcc agccacgatg gcggcaagca ggcgctggag
     2881 acggtgcagc ggctgttgcc ggtgctgtgc caggcccatg gcctgacccc gcagcaggtg
     2941 gtggccatcg ccagcaatgg cggcggcagg ccggcgctgg agagcattgt tgcccagtta
     3001 tctcgccctg atccggcgtt ggccgcgttg accaacgacc acctcgtcgc cttggcctgc
     3061 ctcggcggac gtcctgcgct ggatgcagtg aaaaagggat tgccgcacgc gccggccttg
     3121 atcaaaagaa ccaatcgccg tattcccgaa cgcacatccc atcgcgttgc cgaccacgcg
     3181 caagtggttc gcgtgctggg ttttttccag tgccactccc acccagcgca agcatttgat
     3241 gacgccatga cgcagttcgg gatgagcagg cacgggttgt tacagctctt tcgcagagtg
     3301 ggcgtcaccg aactcgaagc ccgcagtgga acgctccccc cagcctcgca gcgttgggac
     3361 cgtatcctcc aggcatcagg gatgaaaagg gccaaaccgt cccctacttc aactcaaacg
     3421 ccggatcagg cgtctttgca tgcattcgcc gattcgctgg agcgtgacct tgatgcgcct
     3481 agcccaatgc acgagggaga tcagacgcgg gcaagcagcc gtaaacggtc ccgatcggat
     3541 cgtgctgtca ccggtccctc cgcacagcaa tcgttcgagg tgcgcgttcc cgaacagcgc
     3601 gatgcgctgc atttgcccct cagttggagg gtaaaacgcc cgcgtaccag tatcgggggc
     3661 ggcctcccgg atcctggtac gcccacggct gccgacctgg cagcgtccag caccgtgatg
     3721 cgggaacaag atgaggaccc cttcgcaggg gcagcggatg atttcccggc attcaacgaa
     3781 gaggagctcg catggttgat ggagctattg cctcagtgaa gctttgagac cacgaagtgg
     3841 ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3901 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4021 atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg
     4081 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     4141 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     4201 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     4261 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     4321 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     4381 cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa
     4441 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     4501 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     4561 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     4621 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     4681 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     4741 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     4801 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     4861 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     4921 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     4981 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga
     5041 tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg
     5101 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5341 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatag
//
