LOCUS       sequence-207510-       10595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding glutathione 
            S-transferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_GST
FEATURES             Location/Qualifiers
     source          1..10595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3916..4569
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4527..4549
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     terminator      4617..4804
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4672..4696)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4879..6235
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4879..5222
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5223..6032
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5290..5309)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6038..6235
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6329..6346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6329..6345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6338..6360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9244..10338
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgctcatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa
     3961 cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc
     4021 gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt
     4081 ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata
     4141 gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt
     4201 gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt
     4261 gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat
     4321 cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg
     4381 tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa
     4441 ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc
     4501 agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat
     4561 cctccaaaat cggatctggt tccgcgttga ggcgcgccag atctacttct aaataagcga
     4621 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     4681 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     4741 tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta
     4801 ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg
     4861 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg
     4921 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta
     4981 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc
     5041 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac
     5101 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat
     5161 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa
     5221 ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg
     5281 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     5341 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     5401 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     5461 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     5521 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     5581 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     5641 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     5701 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     5761 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     5821 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     5881 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     5941 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     6001 agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt
     6061 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     6121 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     6181 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     6241 gattcgatac taacgccgcc atccagtcga cgaactagta taacttcgta tatggtatta
     6301 tatacgaagt tatgcggccg ctgaattcac tggccgtcgt tttacaacgt cgtgactggg
     6361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6481 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     6541 catcgtccgg atcaatgaag tagatgagtt tactacctgt atatactttt ttttttgtca
     6601 aagtcacttt tatggcattt ccatgttgaa ggattagcca cagcactaaa ttttcagcgt
     6661 atgattgctt ttaaatattt aattttcatc gttttttaat taatattcaa aacgatttaa
     6721 tgccttctac atctgaagtc aagctcatag actcgggttg ggtagctaaa actacatcta
     6781 acacgaaggg gtatagcctt cttaaagtat actcatgtat aaacgttttg ggttcatatt
     6841 tttgggccac gtggtttata aaaattctta actacaccac tcggtatccc gcacccgtct
     6901 acgtttctac gatttcgagc taatattgag taaagtgatt agcaaaaaaa ataaaagtac
     6961 tcgttgtcgg agatcaagaa tttttctatt atctcatcta aaccactttc taaaagcgaa
     7021 aaacaaaatc gtaatatgca gcttgaatgg gcttccatag tttgaaagaa aaaccctagc
     7081 agtactggca agggagacat tccttttacc tggctaatcg ccatcttcca gcaggcgcac
     7141 cattgcccct gtttcactat ccaggttacg gatatagttc atgacaatat ttacattggt
     7201 ccagccacca gcttgcatga tctccggtat tgaaactcca gcgcgggcca tatctcgcgc
     7261 ggctccgaca cgggcactgt gtccagacca ggccaggtat ctctgaccag agtcatcctt
     7321 agcgccgtaa atcaatcgat gagttgcttc aaaaatccct tccagggcgc gagttgatag
     7381 ctggctggtg gcagatggcg cggcaacacc attttttctg acccggcaaa acaggtagtt
     7441 attcggatca tcagctacac cagagacgga aatccatcgc tcgaccagtt tagttacccc
     7501 caggctaagt gccttctcta cacctgcggt gctaaccagc gttttcgttc tgccaatatg
     7561 gattaacatt ctcccaccgt cagtacgtga gatatcttta accctgatcc tggcaatttc
     7621 ggctatacgt aacagggtgt tataagcaat ccccagaaat gccagattac gtatatcctg
     7681 gcagcgatcg ctattttcca tgagtgaacg aacctggtcg aaatcagtgc gttcgaacgc
     7741 tagagcctgt tttgcacgtt caccggcatc aacgttttct tttcggatcc gccgcataac
     7801 cagtgaaaca gcattgctgt cacttggtcg tggcagcccg gaccgacgat gaagcatgtt
     7861 tagctggccc aaatgttgct ggatagtttt tactgccaga ccgcgcgcct gaagatatag
     7921 aagataatcg cgaacatctt caggttctgc gggaaaccat ttccggttat tcaacttgca
     7981 ccatgccgcc cacgaccggc aaacggacag aagcattttc caggtatgct cagaaaacgc
     8041 ctggcgatcc ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg ttgcatcgac
     8101 cggtaacttt tagattaaca tttgtttaaa tatgaaatta ggcaatgtaa catactgcag
     8161 gcaaattttg gtgtacggta agtaaattgg acatatgatt taacaaagcg actataagtc
     8221 agaaagtgag aatgagattg aaataattaa ttcaacttat tcaattgatg atatgccagg
     8281 attcctcttc ctttatatat cttcaatgtt tcgattaaca ggtccattat ccggttttta
     8341 gtttcgctgc acattgccga atgacaatcg gattccttta agctttgctt ttgccctgtt
     8401 ttctctcccc agtaatcgtt gggtgactga accatccagt agccgagagt tacgtttttc
     8461 ctgatttaat aaatatccat atctatttgt ctcgtggtgt tttacagcaa ctaaccgaaa
     8521 ttactatttt gcatcatcca accataaaga gacaacaata attctagaaa acatataaca
     8581 agtgtttttt tctatttacg tctcatacgc aaagtccact ttaccatgtt ttggaaactt
     8641 gtacccaacc attcattttt cgattttgta atgaggctct tttcttctaa caatcattga
     8701 ggtcattatc atgtgtcctc acctattgtt taaattgagg cttgcgctat tcccaaaatt
     8761 gtgggtatta ttttcatata tcagtatctg caatttcggt tcaactccgg aagctcggta
     8821 cccaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca
     8881 ttattttttt tctctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
     8941 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
     9001 ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat
     9061 agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta
     9121 aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta
     9181 ccttttacat ttcagcaata tatatatata tatatatata tatttcaagg atataccatt
     9241 gtaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc
     9301 acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc
     9361 gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt tccacttcca
     9421 gatgaggcgc tggaagcctc caagaaggct gatgccgttt tgttaggtgc tgtgggtggt
     9481 cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa
     9541 cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta
     9601 tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgttag agaattagtg
     9661 ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt
     9721 gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta
     9781 caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca
     9841 agattatgga gaaaaactgt ggaggaaacc atcaagaacg aatttcctac attgaaagtt
     9901 caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat
     9961 ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc
    10021 ccaggctcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc
    10081 gcatttggtt tgtacgaacc atgccatggt tccgctccag atttgccaaa gaataaggtc
    10141 aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct
    10201 gaagaaggta aagccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact
    10261 ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt
    10321 aagaaaatcc ttgcttaaaa agattctctt tttttgtgat atttgtacat aaactttata
    10381 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga
    10441 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag
    10501 gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact
    10561 ttaacattaa tattgacaag gaggagggca tcaca
//
