LOCUS       sequence-207513-       10466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding the Twin Affinity 
            Purification protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_TAP
FEATURES             Location/Qualifiers
     source          1..10466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3874..3907)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3919..3996
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             4024..4044
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      4488..4675
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4543..4567)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4750..6106
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4750..5093
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5094..5903
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5161..5180)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5909..6106
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6200..6217)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6200..6216)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6209..6231)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9115..10209
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg
     3901 aagttatgca tgcttatgaa gcgacgatgg aaaaagaatt tcatagccgt ctcagcagcc
     3961 aaccgcttta agaaaatctc atcctccggg gcacttgatt atggtacccc aactactgct
     4021 tctgaaaatc tatattttca aggtgaacta aaaactgctg ctttggctca acatgcgatt
     4081 aaagctgatg cgcaacaaaa taacttcaac aaagatcaac aaagcgcctt ctatgaaatc
     4141 ttgaacatgc ctaacttaaa cgaagcgcaa cgtaacggct tcattcaaag tcttaaagac
     4201 gacccaagcc aaagcactaa cgttttaggt gaagctaaaa aattaaacga atctcaagca
     4261 ccgaaagctg ataacaattt caacaaagaa caacaaaatg ctttctatga aatcttgaat
     4321 atgcctaact taaacgaaga acaacgcaat ggtttcatcc aaagcttaaa agatgaccca
     4381 agccaaagtg ctaacctatt gtcagaagct aaaaagttaa atgaatctca agcaccgaaa
     4441 gcggataaca aattcaacaa agaataaggc gcgccacttc taaataagcg aatttcttat
     4501 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     4561 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     4621 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat
     4681 ccgctaggga taacagggta atatagatct gtttagcttg cctcgtcccc gccgggtcac
     4741 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
     4801 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
     4861 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag
     4921 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
     4981 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
     5041 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta
     5101 aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg
     5161 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
     5221 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     5281 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     5341 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggcaaaa
     5401 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     5461 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     5521 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     5581 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
     5641 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     5701 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     5761 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     5821 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     5881 tgatgctcga tgagtttttc taatcagtac tgacaataaa aagattcttg ttttcaagaa
     5941 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     6001 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     6061 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     6121 ctaacgccgc catccagtcg acgaactagt ataacttcgt atatggtatt atatacgaag
     6181 ttatgcggcc gctgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6241 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6301 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     6361 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata ccatcgtccg
     6421 gatcaatgaa gtagatgagt ttactacctg tatatacttt tttttttgtc aaagtcactt
     6481 ttatggcatt tccatgttga aggattagcc acagcactaa attttcagcg tatgattgct
     6541 tttaaatatt taattttcat cgttttttaa ttaatattca aaacgattta atgccttcta
     6601 catctgaagt caagctcata gactcgggtt gggtagctaa aactacatct aacacgaagg
     6661 ggtatagcct tcttaaagta tactcatgta taaacgtttt gggttcatat ttttgggcca
     6721 cgtggtttat aaaaattctt aactacacca ctcggtatcc cgcacccgtc tacgtttcta
     6781 cgatttcgag ctaatattga gtaaagtgat tagcaaaaaa aataaaagta ctcgttgtcg
     6841 gagatcaaga atttttctat tatctcatct aaaccacttt ctaaaagcga aaaacaaaat
     6901 cgtaatatgc agcttgaatg ggcttccata gtttgaaaga aaaaccctag cagtactggc
     6961 aagggagaca ttccttttac ctggctaatc gccatcttcc agcaggcgca ccattgcccc
     7021 tgtttcacta tccaggttac ggatatagtt catgacaata tttacattgg tccagccacc
     7081 agcttgcatg atctccggta ttgaaactcc agcgcgggcc atatctcgcg cggctccgac
     7141 acgggcactg tgtccagacc aggccaggta tctctgacca gagtcatcct tagcgccgta
     7201 aatcaatcga tgagttgctt caaaaatccc ttccagggcg cgagttgata gctggctggt
     7261 ggcagatggc gcggcaacac cattttttct gacccggcaa aacaggtagt tattcggatc
     7321 atcagctaca ccagagacgg aaatccatcg ctcgaccagt ttagttaccc ccaggctaag
     7381 tgccttctct acacctgcgg tgctaaccag cgttttcgtt ctgccaatat ggattaacat
     7441 tctcccaccg tcagtacgtg agatatcttt aaccctgatc ctggcaattt cggctatacg
     7501 taacagggtg ttataagcaa tccccagaaa tgccagatta cgtatatcct ggcagcgatc
     7561 gctattttcc atgagtgaac gaacctggtc gaaatcagtg cgttcgaacg ctagagcctg
     7621 ttttgcacgt tcaccggcat caacgttttc ttttcggatc cgccgcataa ccagtgaaac
     7681 agcattgctg tcacttggtc gtggcagccc ggaccgacga tgaagcatgt ttagctggcc
     7741 caaatgttgc tggatagttt ttactgccag accgcgcgcc tgaagatata gaagataatc
     7801 gcgaacatct tcaggttctg cgggaaacca tttccggtta ttcaacttgc accatgccgc
     7861 ccacgaccgg caaacggaca gaagcatttt ccaggtatgc tcagaaaacg cctggcgatc
     7921 cctgaacatg tccatcaggt tcttgcgaac ctcatcactc gttgcatcga ccggtaactt
     7981 ttagattaac atttgtttaa atatgaaatt aggcaatgta acatactgca ggcaaatttt
     8041 ggtgtacggt aagtaaattg gacatatgat ttaacaaagc gactataagt cagaaagtga
     8101 gaatgagatt gaaataatta attcaactta ttcaattgat gatatgccag gattcctctt
     8161 cctttatata tcttcaatgt ttcgattaac aggtccatta tccggttttt agtttcgctg
     8221 cacattgccg aatgacaatc ggattccttt aagctttgct tttgccctgt tttctctccc
     8281 cagtaatcgt tgggtgactg aaccatccag tagccgagag ttacgttttt cctgatttaa
     8341 taaatatcca tatctatttg tctcgtggtg ttttacagca actaaccgaa attactattt
     8401 tgcatcatcc aaccataaag agacaacaat aattctagaa aacatataac aagtgttttt
     8461 ttctatttac gtctcatacg caaagtccac tttaccatgt tttggaaact tgtacccaac
     8521 cattcatttt tcgattttgt aatgaggctc ttttcttcta acaatcattg aggtcattat
     8581 catgtgtcct cacctattgt ttaaattgag gcttgcgcta ttcccaaaat tgtgggtatt
     8641 attttcatat atcagtatct gcaatttcgg ttcaactccg gaagctcggt acccaactgt
     8701 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt
     8761 ttctctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
     8821 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
     8881 tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg
     8941 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat
     9001 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca
     9061 tttcagcaat atatatatat atatatatat atatttcaag gatataccat tgtaatgtct
     9121 gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa
     9181 gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa
     9241 aatcatttaa ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg
     9301 ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg
     9361 ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg
     9421 tacgccaact taagaccatg taactttgca tccgactctc ttttagactt atctccaatc
     9481 aagccacaat ttgctaaagg tactgacttc gttgttgtta gagaattagt gggaggtatt
     9541 tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac
     9601 accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag
     9661 ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg
     9721 agaaaaactg tggaggaaac catcaagaac gaatttccta cattgaaagt tcaacatcaa
     9781 ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata
     9841 atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggctcc
     9901 ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt
     9961 ttgtacgaac catgccatgg ttccgctcca gatttgccaa agaataaggt caaccctatc
    10021 gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt
    10081 aaagccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta
    10141 ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc
    10201 cttgcttaaa aagattctct ttttttgtga tatttgtaca taaactttat aaatgaaatt
    10261 cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat
    10321 atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg
    10381 taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta
    10441 atattgacaa ggaggagggc atcaca
//
