LOCUS       sequence-207517-       10366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10366)
  TITLE     William Hahn plasmids
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPs-neo HA-RalA
FEATURES             Location/Qualifiers
     source          1..10366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     CDS             1039..1065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2050..2070)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2068..2365
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2724..3518
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2778..2797)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3388..3407
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          3938..4003
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4133..4153
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7278..7296)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7279..7296)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7586..7605)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7998..8016)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8084..8188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8189..8257,8258..9049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8407..8426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9220..9808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9709..9728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9962..9979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10096..10117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10132..10137,10138..10155,10156..10162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10170..10186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10175..10197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10194..10210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10194..10210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgtctgta
      721 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
      781 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
      841 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
      901 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
      961 ccgcgcgtct tgtctgctgc aggtcgacaa gcttggtacc gagctcggat cgattaatta
     1021 accgcggtca ccatgggtta cccttacgac gtgccagact atgcctccgg tccgatggct
     1081 gcaaataagc ccaagggtca aaattctttg gccttacaca aagtcatcat ggtgggcagt
     1141 gttggtgtgg gcaagtcagc tctgactcta cagttcatgt acgatgagtt tgtggaggac
     1201 tatgagccta ccaaagcaga cagctaccgg aagaaggtag tgctggatgg ggaggaagta
     1261 cagattgata tcttagatac agctgggcag gaggactatg ctgcaattag agacaactac
     1321 ttccgaagcg gggagggctt cctctgtgtc ttctctatta cagaaatgga atcctttgca
     1381 gctacagctg acttcaggga gcagatttta agagtaaaag aagacgagaa tgttccattt
     1441 ctactggttg gtaacaaatc agatttagaa gataaaaggc aggtttctgt agaagaggca
     1501 aaaaacagag ctgaccagtg gaatgttaac tacgtggaaa cgtctgctaa aacacgagct
     1561 aatgttgaca aggtattttt tgatttaatg agagaaattc gagcaagaaa gatggaagac
     1621 agcaaagaaa agaatggaaa aaagaagagg aaaagtttag ccaagagaat cagagaaaga
     1681 tgctgcattt tataacggtc cggatccccg ggcgagctcg aattcagatc agcttttgtt
     1741 tctgtttatt ttttatggga ccccttattg tactcctaat gattttgctc ttcggaccct
     1801 gcattcttaa tcgtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1861 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     1921 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1981 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     2041 ggcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2101 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2161 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2221 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2281 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2341 agaagtagtg aggaggcttt tttggaggag cttcacgctg ccgcaagcac tcagggcgca
     2401 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     2461 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     2521 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     2581 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     2641 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag
     2701 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     2761 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     2821 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     2881 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     2941 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3001 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3061 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3121 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3181 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3241 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3301 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3361 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3421 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3481 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     3541 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     3601 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3661 ggatctcatg ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag
     3721 ctgctgcctg aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat
     3781 ccaggaaacc agcagcggct atccgcgcat ccatgccccc gaactgcagg agtggggagg
     3841 cacgatggcc gctttggtcc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     3901 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     3961 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     4021 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     4081 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     4141 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     4201 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     4261 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     4321 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     4381 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     4441 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     4501 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     4561 atgcaattgt tgttgttcat cgatggatcg atccgattag tccaatttgt taaagacagg
     4621 atatcagtgg tccaggctct agctttgact caacaatatc accagctgaa gcctatagag
     4681 tacgagccat agttaaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     4741 gaccccaccc gtaggtggca agctagctta agtaacgcca ctttgcaagg catggaaaaa
     4801 tacataactg agaatagaaa agttcagatc aaggtcagga acaaagaaac agctgaatac
     4861 caaacaggat atctgtggta agcggttcct gccccggctc agggccaaga acagatgaga
     4921 cagctgagtg atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcgggg
     4981 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctagt gaatcatcag
     5041 atgtttccag ggtgccccaa ggacctgaaa atgaccctgt accttatttg aactaaccaa
     5101 tcagttcgct tctcgcttct gttcgcgcgc ttccgctctc cgagctcaat aaaagagccc
     5161 acaacccctc actcggcgcg ccagtcttcc gatagactgc gtcgcccggg tacccgtatt
     5221 cccaataaag cctcttgctg tttgcatccg aatcgtggtc tcgctgttcc ttgggagggt
     5281 ctcctctgag tgattgacta cccacgacgg gggtctttca ctttttcttt tagtttgtgg
     5341 atgctttcta gagttccagg tgagaaaata agaagcaaca aaaataagag tcatttgaaa
     5401 aaaaaaacaa aaaaaacctg tcgcgtttcc tagactatct ggaagctagc ttcgacacag
     5461 actccagctt cagtcccttc ccagttccgc ccctaattcc tttcccaagg gggaggagtc
     5521 tatcttttca gacccagttc gtggggtgac gtcccaggaa aatttctact gcattcccct
     5581 ctcgcacctt cccctcttcc cgcagtcgcc tccactctcc tcaccgtccc catcctcatc
     5641 catggcgaaa tcttcgcccc ccgcctcgtg aagatccagc acgtccgcca tcttgcttct
     5701 ctcggcgctt tctgagtctc tagaagctaa gcacctcggg aagctgtcgc agaagaaaaa
     5761 cgtcacgagt taggctgacc aattaaaggt tgcctccaag tcccgccctc ggcgcggtga
     5821 aaattcgcca atgtaggcgg ccccactatg aagcgctaga aacgtccagc actctgggac
     5881 cccgatggag ctctgcgagt atgaggccgt aggctacatg gtttgtgatt gtgttttgtt
     5941 ttcttttctt ttgaggcagg gtctctctat atagccctag ctgggctcga agttacagat
     6001 atccaactat attccccttc cacatgctgc tgggattaaa agcctgtgct accacgccgg
     6061 gctccaaggc tacagaatga gaccctttct caaaaacata aaaagtaaaa taaagtggtt
     6121 tcggaagttc tacagccaag ttgatcaggt agattgaagg tccggagcgc agccgcgttg
     6181 gggcaaattt aaagggtgag tgaagtcagt gactttctca taaaactttc aactagcaag
     6241 aaatcctttg atacatatct acatatatgg gtttattgaa atgacttacg ggcccagcta
     6301 attggagaat aactagatgt gtccagaaag tcgaagaatc tagtatttgc ttagttccac
     6361 aaggctggat gtctcagctg gtctagaatc cagaaaaagt aggctccagt gccggtgaag
     6421 aaacggactt atgagcaagg caaagtgcaa aagtttcctt catccatgtt tttatatagg
     6481 cttcagcaaa aggtgtggcc caggttaaag gtgtgcctgc ccgctttaat cccagcactt
     6541 gggaggcaga ggcaggcagc tttctgagtt cgaggtcagc ctggtctaca gagtgagttc
     6601 caggacagcc agggctacac agagaaaccc tgtctccaaa aacaaaaaca aaacaaacaa
     6661 acaaaagatt aaagaaaagc ctgtgtcttc ctgcctctag atgtagctca gaggtgttct
     6721 ttcctaccta aagtctggac tagaagtgga tctacccact ccaccaaaca gaaaatcaca
     6781 ggtgtgcccc tcatttctgg atttgtagtt cattccagag agagtaagcc tgacaactga
     6841 gaagaaccat ctcaggtgtt tttgtttttg tttttttttc ttttcaatac agggtttctc
     6901 tgtgtagctc tgggtgtcct ggaactcact ctgtatttcg aggctgtcct cgatctcaga
     6961 aatccgcctg tctctgcctc ccaagtgctg ggattaaagg catgtgccac cacttccccg
     7021 ctcaggtggt tttttttgtt tgtttatttt ttatttattt gagtacactg tagctgtctt
     7081 cagacacatt agaagagggc atcagatccc attacagatg gttgtaaacc accatgtggt
     7141 tgctgggatt tgaactcagg acctccagaa gagcagtccg tgcgcttaac cactgagcca
     7201 tctctccaac ccagtttggt ttttttttgt tgttgttttt tttttttggg ggggggaatt
     7261 aattccggag cttgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     7321 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     7381 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     7441 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     7501 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     7561 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     7621 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     7681 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     7741 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     7801 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     7861 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7921 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7981 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     8041 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8101 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8161 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8221 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8281 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8341 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8401 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8461 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8521 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8581 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8641 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8701 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8761 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8821 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8881 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8941 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9001 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9061 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9121 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9361 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9601 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9781 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9841 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9901 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9961 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    10021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    10081 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    10141 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    10201 cagctatgac atgattacga attaattcat accagatcac cgaaaactgt cctccaaatg
    10261 tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc
    10321 cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct
//
