LOCUS       sequence-207522-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TRMT10C (RG9MTD1A-c113)
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             610..630
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             649..672
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcaacctt tggtttttga catggcttac gaaaattata tgaaacgaaa agaattgcag
       61 aatactgttt cccagctttt agaaagtgaa ggatggaaca gaagaaatgt tgatcctttc
      121 catatttatt tctgcaatct aaaaatagat ggtgctttgc acagagagtt agttaaacgg
      181 tatcaagaaa aatgggacaa attgctttta acatcaacag aaaagtctca tgtagattta
      241 tttccaaagg acagtattat ctatttaact gcagattctc ccaatgttat gactactttc
      301 aggcatgaca aagtttatgt aattgggtct tttgttgata agagtatgca gccaggcaca
      361 tccctagcca aggcaaaacg gctgaacctg gcaactgaat gccttccatt agataaatat
      421 ttacaatggg aaattggtaa caaaaatctc accttagatc aaatgatacg tattttgtta
      481 tgtctgaaaa acaatggtaa ttggcaagag gctctgcaat tcgttcccaa gagaaaacat
      541 actggttttc tggagatttc tcagcattct caagagttta tcaacagact aaagaaggca
      601 aagactgcag agaacctcta cttccaatcg caccatcatc accaccatga ttacaaggat
      661 gacgacgata agtgaggatc c
//
