LOCUS       sequence-207524-         385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 385)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WNK2 (WNK2A-c003)
FEATURES             Location/Qualifiers
     source          1..385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cagaggacac aggcgtgagg gtggagctcg cggaggagga ccacggcagg
      121 aagtccacca tcgccctgag gctctgggtg gaagacccca agaaactgaa gggaaagccc
      181 aaggacaatg gagccataga gttcaccttc gacctggaga aggagacgcc ggatgaggtg
      241 gcccaagaga tgattgagtc tggattcttc cacgagagtg acgtcaagat cgtggccaag
      301 tccatccgtg accgcgtggc cttgatccag tggcggcggg agaggatctg gcccgcgtga
      361 cagtaaaggt ggatacggat ccgaa
//
