LOCUS       sequence-207525-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KLHL3 (KLHL3A-c003)
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gccttcccaa ggtcatgatt gtggttggcg gccaggcacc caaggcaatc
      121 cgcagtgtgg agtgctatga tttcgaggag gaccggtggg atcagattgc tgagcttcct
      181 tccagaagat gcagagcagg tgtggtgttc atggctggcc acgtgtatgc cgtgggaggg
      241 tttaatggct cactgcgggt gcggacagtg gatgtgtatg acggcgtgaa ggaccagtgg
      301 acgtccattg ccagcatgca ggagcgccgg agcacactgg gcgcagcggt gctcaatgac
      361 ttgctctacg cagtgggagg ctttgatggc agtactggcc tagcatcggt ggaagcctac
      421 agctacaaga ccaacgagtg gttctttgtg gccccgatga acacgcggcg gagcagtgtg
      481 ggtgtgggcg ttgtggaggg gaagctatat gctgttgggg gttatgatgg agcttcccgc
      541 cagtgtctga gcactgtgga gcagtacaac ccagcgacca atgaatggat atacgtggcg
      601 gacatgagca cccgccgcag tggcgcaggg gttggagtgc ttagcggaca gctgtacgcc
      661 acaggtgggc atgatgggcc tttggtgagg aagagcgttg aggtttacga tcctggaaca
      721 aatacctgga agcaagtggc agacatgaac atgtgccggc gcaacgcagg ggtctgtgca
      781 gtaaatgggc tcctgtatgt ggttggaggg gatgatggat cctgcaactt ggcttcggtg
      841 gagtactaca atcctgtcac tgacaaatgg acgctgcttc caacgaacat gagcacgggg
      901 cggagctatg caggtgttgc cgtgattcac aagtccttgt gacagtaaag gtggatacgg
      961 atccgaa
//
