LOCUS       sequence-207526-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WNK3 (WNK3B-c016)
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cagaaatgaa ggctgtagct acttctccta gtggcagatt cctgaaattt
      121 gacatagaac taggaagagg agcatttaaa acagtatata aaggactgga cactgaaaca
      181 tgggttgagg ttgcttggtg tgagctgcag gaccgaaagt taaccaaagc tgagcagcaa
      241 agattcaagg aagaagcaga gatgttgaag ggtctccagc accccaatat agttcgattt
      301 tatgattcct gggaatctat attaaaagga aagaaatgta ttgtattagt gactgaacta
      361 atgacatctg ggaccttaaa gacgtactta aaacgattta aagtcatgaa accaaaggtc
      421 ttaaggagct ggtgcaggca aattttaaag gggttgcagt tcttgcacac taggactcct
      481 cctattattc accgggatct gaagtgtgac aatattttca tcacgggacc cactggatct
      541 gtgaagattg gtgatctagg attagccacc ttaatgcgta cctcatttgc taagagtgtc
      601 attggaactc ctgagtttat ggctccagag atgtatgaag aacactatga tgaatccgta
      661 gatgtttatg cttttggaat gtgtatgctg gaaatggcca catcggagta tccttattct
      721 gagtgtcaga atgcagctca aatataccgg aaagtaacta gtggcataaa accagccagc
      781 ttcaataaag tcactgatcc tgaagtcaaa gaaatcattg aaggatgtat tcgtcaaaac
      841 aaatctgaaa ggttgtctat cagggaccta ttaaaccatg cattttttgc tgaggataca
      901 ggactgaggg tggagtgaca gtaaaggtgg atacggatcc gaa
//
