LOCUS       sequence-207527-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CHD1 (CHD1A-c107)
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc ctcgggagaa tattaaagga tttagtgatg cagaaattag gcggtttatc
      121 aagagctata agaaatttgg tggtcctctg gaaagattag atgcaattgc tcgagatgct
      181 gagttagttg ataagtcaga aacagacctt agacgactgg gagaattggt acataatggt
      241 tgcattaaag cattaaagga tagttcttca ggaacagaac gaacaggtgg tagactcgga
      301 aaagtgaagg gtccaacatt ccgaatatca ggagtacagg tgaatgccaa actagtcatc
      361 tcccatgaag aagaattaat acctttgcac aaatccattc cttctgatcc agaagaaaga
      421 aagcagtata ctatcccatg ccacacaaag gcagctcatt ttgatataga ctggggcaaa
      481 gaagatgatt ccaatttgtt aattggcatc tatgaatatg gatatggaag ctgggaaatg
      541 attaaaatgg atcctgacct cagtctaaca cacaagattc ttccagatga tcccgataaa
      601 aaaccacaag caaaacagtt gcagacccgt gcagactacc tcatcaaatt acttagtaga
      661 gatcttgcaa aaaaagaagc tctttctggt gcgggatgac agtaaaggtg gatacggatc
      721 cgaa
//
