LOCUS       sequence-207532-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ARF1 (ARF1A-c001)
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..42
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             79..102
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             127..147
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgtcttctg gtgcgagctg gagtcaccca caattcgaga aaggtggtgg ctctggtggc
       61 ggttcaggcg gagccgcctg gtcccatcca cagtttgaaa agggttctgg tgtagatctg
      121 ggtaccgaga acctgtactt ccaatccatg cgcattctga tggttggctt agatgcagcg
      181 ggtaaaacca cgattctgta caaactgaaa ctgggcgaga ttgtgacgac gattccgacc
      241 attgggttca acgtcgaaac cgtggagtac aagaacatct cgtttaccgt atgggatgtt
      301 ggtggacaag acaagattcg tccattgtgg cgccattatt tccagaacac tcaaggcctg
      361 atctttgtgg tcgacagcaa tgaccgtgaa cgggttaacg aagctcgtga agagctgatg
      421 cgcatgttag ccgaagatga actgcgcgat gcggtactgc tcgtgtttgc caacaaacag
      481 gatctgccga atgcgatgaa tgctgccgaa atcaccgaca aacttggcct tcactctttg
      541 cgtcatcgca actggtatat ccaggcaact tgcgcgacaa gtggtgatgg gctctatgaa
      601 ggtctggatt ggctgtccaa tcagttacgc aatcagaaat gac
//
