LOCUS       sequence-207533-        1219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1219)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IQSEC2 (IQSEC2A-c001)
FEATURES             Location/Qualifiers
     source          1..1219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..33
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca tcatcatcat cattcttctg gtgtagatct gggtaccgag
       61 aacctgtact tccaatccat gtgcaaacag acctatcagc gtgaaacccg tcatagctgg
      121 gatagtccgg catttaacaa tgatgttgtt cagcgtcgtc attatcgtat tggtctgaac
      181 ctgtttaaca agaaaccgga aaaaggcatc cagtatctga ttgaacgtgg ttttctgagc
      241 gatacaccgg ttggtgttgc acattttatt ctggaacgta aaggtctgag ccgtcagatg
      301 attggtgaat ttctgggtaa tcgtcagaaa cagtttaatc gtgatgttct ggattgcgtg
      361 gttgatgaaa tggattttag cagcatggat ctggatgatg cactgcgtaa atttcagagc
      421 catattcgtg ttcagggtga agcacagaaa gttgaacgcc tgattgaagc atttagccag
      481 cgttattgtg tttgtaatcc ggcactggtt cgtcagtttc gcaatccgga taccattttt
      541 atcctggcct ttgcaattat tctgctgaac accgatatgt atagcccgag cgttaaagca
      601 gaacgtaaaa tgaaactgga cgacttcatt aaaaacctgc gtggtgttga taacggtgaa
      661 gatattccgc gtgatctgct ggttggtatt tatcagcgta ttcagggtcg tgaactgcgt
      721 accaatgatg atcatgttag ccaggttcag gcagttgaac gtatgattgt tggtaaaaaa
      781 ccggttctga gcctgccgca tcgtcgtctg gtttgttgtt gtcagctgta tgaagttccg
      841 gatccgaatc gtccgcagcg tctgggtctg catcagcgcg aagtttttct gtttaatgat
      901 ttactggtgg tgaccaaaat cttccagaaa aaaaagatcc tggtgaccta tagctttcgt
      961 cagagctttc cgctggttga aatgcacatg cagctgtttc agaacagcta ttatcagttt
     1021 ggcattaaac tgctgagcgc agttccaggt ggtgaacgca aagttctgat tatctttaat
     1081 gcaccgagcc tgcaggatcg tctgcgtttt accagcgatc tgcgtgaaag cattgccgaa
     1141 gttcaagaga tggaaaaata tcgtgttgaa agcgagctgg aaaaacagaa atgacagtaa
     1201 aggtggatac ggatccgaa
//
