LOCUS       sequence-207537-        1997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1997)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DPYSL4 (DPYSL4A-c003)
FEATURES             Location/Qualifiers
     source          1..1997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..204
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgggccacc atcatcatca tcattcttct ggtgtggata acaagttcaa caaggagcgt
       61 cgaagagctc gccgtgaaat tcgccatctg ccgaacctga accgcgaaca gcgtcgcgca
      121 tttattcgca gcctgcgcga tgatccgagc cagagcgcga acctgctggc ggaagcgaag
      181 aagctgaacg atgcgcagcc gaagggtaca gagaacctgt acttccaatc catgtccttc
      241 cagggcaaga aaagcatccc ccggatcacg agtgaccgcc ttctgatcag aggtgggagg
      301 atcgtgaatg acgaccagtc cttttacgct gatgtgcacg tggaagatgg cttgataaaa
      361 caaatcggag aaaacctcat cgtccctggg ggcatcaaga ccattgacgc ccacggcctg
      421 atggtccttc ctggtggcgt tgacgtccac acaaggctgc agatgcctgt cctgggcatg
      481 acaccggctg acgacttctg tcagggcacc aaggcagcgc tagcaggagg aaccaccatg
      541 atcttggacc acgtcttccc cgacacgggt gtgagcctgc tggcggccta cgagcagtgg
      601 cgggagcggg cggacagcgc ggcctgctgc gactactccc tgcacgtgga catcacccga
      661 tggcatgaga gcatcaagga ggagctggag gccctggtca aggagaaggg tgtgaactcc
      721 ttcctggtct tcatggcata caaggaccgg tgccagtgca gcgacagcca gatgtacgag
      781 atcttcagca tcatccggga cctgggggcc ttggcccagg tgcacgctga gaacggggac
      841 atcgtggagg aggagcagaa gcggttgctg gagctcggca tcactggccc cgagggccac
      901 gtgctcagcc accccgagga ggtggaggct gaggcggtgt accgagctgt caccatcgcc
      961 aagcaggcaa actgcccgct gtacgtcacc aaggtgatga gcaagggggc ggccgacgcc
     1021 atcgctcagg ccaagcgcag aggggtggtc gtgtttgggg agcccatcac cgccagcctg
     1081 ggcaccgacg gttcacacta ctggagcaag aactgggcca aggccgcagc cttcgtcaca
     1141 tcaccccctg tcaacccaga ccccaccacg gcagaccacc tcacctgctt gctgtccagc
     1201 ggggacctcc aggtgacagg cagcgcccac tgcaccttca ccactgccca gaaggctgtg
     1261 ggcaaggaca acttcgcgct gatccccgag ggcaccaacg gcattgagga gcgcatgtcg
     1321 atggtctggg agaaatgtgt ggcctctggg aagatggacg agaatgagtt cgtcgcggtg
     1381 accagtacaa atgctgccaa aatcttcaat ttttacccaa ggaaggggcg agtggctgtg
     1441 ggctctgacg ctgacctggt catatggaac cccaaggcca ccaagatcat ctctgccaag
     1501 acccacaatc tgaacgtgga gtacaacatc ttcgagggag tggagtgccg gggagcgcct
     1561 gccgtggtca taagtcaggg ccgagtggcg ctggaggacg ggaagatgtt tgtcaccccg
     1621 ggggcgggcc gcttcgtccc tcggaaaaca ttcccggact ttgtctacaa gaggatcaaa
     1681 gctcgcaaca ggctggcgga gatccacggt gtgccccgtg ggctgtatga cgggcccgtc
     1741 cacgaggtga tggtgcctgc caagccaggg agtggcgctc cggcccgcgc gtcctgccca
     1801 ggcaagatct ccgtccctcc tgtgcgcaac ctacatcagt cggggttcag cctatctggg
     1861 tctcaggctg atgaccacat cgcccgacgc acagcacaga agatcatggc accacctggc
     1921 ggccgctcca acatcacctc tctctcctga cagtaaaggt ggatacggat ccgaattcga
     1981 gctccgtcga caagctt
//
