LOCUS       sequence-207539-        1301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1301)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PKN2 (PRKCL2A-c038)
FEATURES             Location/Qualifiers
     source          1..1301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..204
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgggccacc atcatcatca tcattcttct ggtgtggata acaagttcaa caaggagcgt
       61 cgaagagctc gccgtgaaat tcgccatctg ccgaacctga accgcgaaca gcgtcgcgca
      121 tttattcgca gcctgcgcga tgatccgagc cagagcgcga acctgctggc ggaagcgaag
      181 aagctgaacg atgcgcagcc gaagggtaca gagaacctgt acttccaatc catgtctcag
      241 caaaggtttc agtttaatct acaagatttc aggtgttgtg ctgtcttggg aagaggacat
      301 tttggaaagg tgcttttagc tgaatataaa aacacaaatg agatgtttgc tataaaagcc
      361 ttaaagaaag gagatattgt ggctcgagat gaagtagaca gcctgatgtg tgaaaaaaga
      421 atttttgaaa ctgtgaatag tgtaaggcat ccctttttgg tgaacctttt tgcatgtttc
      481 caaaccaaag agcatgtttg ctttgtaatg gaatatgctg ccggtgggga cctaatgatg
      541 cacattcata ctgatgtctt ttctgaacca agagctgtat tttatgctgc ttgtgtagtt
      601 cttgggttgc agtatttaca tgaacacaaa attgtttata gagatttgaa attggataac
      661 ttattgctag atacagaggg ctttgtgaaa attgctgatt ttggtctttg caaagaagga
      721 atgggatatg gagatagaac aagcacattt tgtggcactc ctgaatttct tgccccagaa
      781 gtattaacag aaacttctta tacaagggct gtagattggt ggggccttgg cgtgcttata
      841 tatgaaatgc ttgttggtga gtctcccttt cctggtgatg atgaagagga agtttttgac
      901 agtattgtaa atgatgaagt aaggtatcca aggttcttat ctacagaagc catttctata
      961 atgagaaggc tgttaagaag aaatcctgaa cggcgccttg gggctagcga gaaagatgca
     1021 gaggatgtaa aaaagcaccc atttttccgg ctaattgatt ggagcgctct gatggacaaa
     1081 aaagtaaagc caccatttat acctaccata agaggacgag aagatgttag taattttgat
     1141 gatgaattta cctcagaagc acctattctg actccacctc gagaaccaag gatactttcg
     1201 gaagaggagc aggaaatgtt cagagatttt gactacattg ctgattggtg ttgacagtaa
     1261 aggtggatac ggatccgaat tcgagctccg tcgacaagct t
//
