LOCUS       sequence-207544-        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DENND1A (DENND1AA-c032)
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1174..1194
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1195..1224
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1225..1248
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggctcca ggatcaagca gaatccagag accacatttg aagtatatgt tgaagtggcc
       61 tatcccagga caggtggcac tctttcagat cctgaggtgc agaggcaatt cccggaggac
      121 tacagtgacc aggaagttct acagactttg accaagtttt gtttcccctt ctatgtggac
      181 agcctcacag ttagccaagt tggccagaac ttcacattcg tgctcactga cattgacagc
      241 aaacagagat tcgggttctg ccgcttatct tcaggagcga agagctgctt ctgtatctta
      301 agctatctcc cctggttcga ggtattttat aagctgctta acatcctggc agattacacg
      361 acaaaaagac aggaaaatca gtggaatgag cttcttgaaa ctctgcacaa acttcccatc
      421 cctgacccag gagtgtctgt ccatctcagc gtgcattctt attttactgt gcctgatacc
      481 agagaacttc ccagcatacc tgagaataga aatctgacag aatattttgt ggctgtggat
      541 gttaacaaca tgttgcatct gtacgccagt atgctgtacg aacgccggat actcatcatt
      601 tgcagcaaac tcagcactct gactgcctgc atccacgggt ctgcggcgat gctctacccc
      661 atgtactggc agcacgtgta catccccgtg ctgccgccgc atctgctgga ctactgctgt
      721 gctcccatgc cctacctcat aggaatccat ttaagtttaa tggagaaagt cagaaacatg
      781 gccctggatg atgtcgtgat cctgaatgtg gacaccaaca ccctggaaac ccccttcgat
      841 gacctccaga gcctcccaaa cgacgtgatc tcttccctga agaacaggct gaaaaaggtc
      901 tccacaacca ctggggatgg tgtggccaga gcgttcctca aggcccaggc tgctttcttc
      961 ggtagctacc gaaacgctct gaaaatcgag ccggaggagc cgatcacttt ctgtgaggaa
     1021 gccttcgtgt cccactaccg ctccggagcc atgaggcagt tcctgcagaa cgccacacag
     1081 ctgcagctct tcaagcagtt tattgatggt cgattagatc ttctcaattc cggcgaaggt
     1141 ttcagtgatg tttttgaaga ggaaatcaac gcagagaacc tctacttcca atcgcaccat
     1201 catcaccatc accatcacca ccatgattac aaggatgacg acgataagtg aggatcc
//
