LOCUS       sequence-207545-        1911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1911)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MTHFR (MTHFRA-c042)
FEATURES             Location/Qualifiers
     source          1..1911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1828..1848
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1849..1878
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1879..1902
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggaccctg agcggcatga gagactccgg gagaagatga ggcggcgatt ggaatctggt
       61 gacaagtggt tctccctgga attcttccct cctcgaactg ctgagggagc tgtcaatctc
      121 atctcaaggt ttgaccggat ggcagcaggt ggccccctct acatagacgt gacctggcac
      181 ccagcaggtg accctggctc agacaaggag acctcctcca tgatgatcgc cagcaccgcc
      241 gtgaactact gtggcctgga gaccatcctg cacatgacct gctgccgtca gcgcctggag
      301 gagatcacgg gccatctgca caaagctaag cagctgggcc tgaagaacat catggcgctg
      361 cggggagacc caataggtga ccagtgggaa gaggaggagg gaggcttcaa ctacgcagtg
      421 gacctggtga agcacatccg aagtgagttt ggtgactact ttgacatctg tgtggcaggt
      481 taccccaaag gccaccccga agcagggagc tttgaggctg acctgaagca cttgaaggag
      541 aaggtgtctg cgggagccga tttcatcatc acgcagcttt tctttgaggc tgacacattc
      601 ttccgctttg tgaaggcatg caccgacatg ggcatcactt gccccatcgt ccccgggatc
      661 tttcccatcc agggctacca ctcccttcgg cagcttgtga agctgtccaa gctggaggtg
      721 ccacaggaga tcaaggacgt gattgagcca atcaaagaca acgatgctgc catccgcaac
      781 tatggcatcg agctggccgt gagcctgtgc caggagcttc tggccagtgg cttggtgcca
      841 ggcctccact tctacaccct caaccgcgag atggctacca cagaggtgct gaagcgcctg
      901 gggatgtgga ctgaggaccc caggcgtccc ctaccctggg ctctcagtgc ccaccccaag
      961 cgccgagagg aagatgtacg tcccatcttc tgggcctcca gaccaaagag ttacatctac
     1021 cgtacccagg agtgggacga gttccctaac ggccgctggg gcaattcctc ttcccctgcc
     1081 tttggggagc tgaaggacta ctacctcttc tacctgaaga gcaagtcccc caaggaggag
     1141 ctgctgaaga tgtgggggga ggagctgacc agtgaagcaa gtgtctttga agtctttgtt
     1201 ctttacctct cgggagaacc aaaccggaat ggtcacaaag tgacttgcct gccctggaac
     1261 gatgagcccc tggcggctga gaccagcctg ctgaaggagg agctgctgcg ggtgaaccgc
     1321 cagggcatcc tcaccatcaa ctcacagccc aacatcaacg ggaagccgtc ctccgacccc
     1381 atcgtgggct ggggccccag cgggggctat gtcttccaga aggcctactt agagtttttc
     1441 acttcccgcg agacagcgga agcacttctg caagtgctga agaagtacga gctccgggtt
     1501 aattaccacc ttgtcaatgt gaagggtgaa aacatcacca atgcccctga actgcagccg
     1561 aatgctgtca cttggggcat cttccctggg cgagagatca tccagcccac cgtagtggat
     1621 cccgtcagct tcatgttctg gaaggacgag gcctttgccc tgtggattga gcagtgggga
     1681 aagctgtatg aggaggagtc cccgtcccgc accatcatcc agtacatcca cgacaactac
     1741 ttcctggtca acctggtgga caatgacttc ccactggaca actgcctctg gcaggtggtg
     1801 gaagacacat tggagcttct caacgcagag aacctctact tccaatcgca ccatcatcac
     1861 catcaccatc accaccatga ttacaaggat gacgacgata agtgaggatc c
//
