LOCUS       sequence-207546-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ULK3 (ULK3A-c002)
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..204
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgggccacc atcatcatca tcattcttct ggtgtggata acaagttcaa caaggagcgt
       61 cgaagagctc gccgtgaaat tcgccatctg ccgaacctga accgcgaaca gcgtcgcgca
      121 tttattcgca gcctgcgcga tgatccgagc cagagcgcga acctgctggc ggaagcgaag
      181 aagctgaacg atgcgcagcc gaagggtaca gagaacctgt acttccaatc catggcgggg
      241 cccggctggg gtcccccgcg cctggacggc ttcatcctca ccgagcgcct gggcagcggc
      301 acgtacgcca cggtgtacaa ggcctacgcc aagaaggaca ctcgtgaagt ggtagccata
      361 aagtgtgtag ccaagaaaag tctgaacaag gcatcggtgg agaacctcct cacggagatt
      421 gagatcctca agggcattcg acatccccac attgtgcagc tgaaagactt tcagtgggac
      481 agtgacaata tctacctcat catggagttt tgcgcagggg gcgacctgtc tcgcttcatc
      541 catacccgca ggattctgcc tgagaaggtg gcgcgtgtct tcatgcagca attagctagc
      601 gccctgcaat tcctgcatga acggaatatc tctcacctgg atctgaagcc acagaacatt
      661 ctactgagct ccttggagaa gccccaccta aaactggcag actttggttt cgcacaacac
      721 atgtccccgt gggatgagaa gcacgtgctc cgtggctccc ccctctacat ggcccccgag
      781 atggtgtgcc agcggcagta tgacgcccgc gtggacctct ggtccatggg ggtcatcctg
      841 tatgaagccc tcttcgggca gccccccttt gcctccaggt cgttctcgga gctggaagag
      901 aagatccgta gcaaccgggt catcgagctc cccttgcggc ccctgctctc ccgagactgc
      961 cgggacctac tgcagcggct cctggagcgg gaccccagcc gtcgcatctc cttccaggac
     1021 ttttttgcgc acccctgggt gtgacagtaa aggtggatac ggatccgaat tcgagctccg
     1081 tcgacaagct t
//
