LOCUS       sequence-207553-        1800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB 
            intergenic region. The Landing Pad consists of floxed mCherry,Chlor 
            that are cotranscribed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S
  TITLE     Systematic Dissection of Sequence Elements Controlling sigma70 
            Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in 
            E. coli.
  JOURNAL   Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069.
  PUBMED    29388765
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069."
COMMENT     Sequence Label: E. coli K-12 MG1655_fLP4(essQ-cspB)
FEATURES             Location/Qualifiers
     source          1..1800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(71..104)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1019..1678
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1085..1104)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cccaaactgc acccaagagt cagaacacag tttttcaaga gtacaaaaag aacacgtccg
       61 tcctagaact taccgttcgt atagcataca ttatacgaag ttataaaaag agtattgact
      121 tcaggaaaat ttttctgcat aattatttca ttactagaat ctcatatatc aaatataggg
      181 tggattacta gatggtttcc aagggcgagg aggataacat ggctatcatt aaagagttca
      241 tgcgcttcaa agttcacatg gagggttctg ttaacggtca cgagttcgag atcgaaggcg
      301 aaggcgaggg ccgtccgtat gaaggcaccc agaccgccaa actgaaagtg actaaaggcg
      361 gcccgctgcc ttttgcgtgg gacatcctga gcccgcaatt tatgtacggt tctaaagcgt
      421 atgttaaaca cccagcggat atcccggact atctgaagct gtcttttccg gaaggtttca
      481 agtgggaacg cgtaatgaat tttgaagatg gtggtgtcgt gaccgtcact caggactcct
      541 ccctgcagga tggcgagttc atctataaag ttaaactgcg tggtactaat tttccatctg
      601 atggcccggt gatgcagaaa aagacgatgg gttgggaggc gtctagcgaa cgcatgtatc
      661 cggaagatgg tgcgctgaaa ggcgaaatta aacagcgcct gaaactgaaa gatggcggcc
      721 attatgacgc tgaagtgaaa accacgtaca aagccaagaa acctgtgcag ctgcctggcg
      781 cgtacaatgt gaatattaaa ctggacatca cctctcataa tgaagattat acgatcgtag
      841 agcaatatga gcgcgcggag ggtcgtcatt ctaccggtgg catggatgag ctgtacaaat
      901 aatactagaa tctcatatat caaatataag gcggattact agatggtttc caagggcatg
      961 gatgagctgt acaaataata ctagaatctc atatatcaaa tataaggcgg attactagat
     1021 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     1081 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     1141 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     1201 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
     1261 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     1321 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     1381 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     1441 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     1501 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     1561 caaggtgttg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     1621 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaata
     1681 gggataacag ggtaatataa cttcgtatat ggtttcttat acgaacggta gaactgagtg
     1741 taccgcttgc gctgtaaaac tattcatgca gcaaacctgc acttcaccac aatggttagc
//
